Recombinant Human CD40L/CD154/TRAP Protein(HEK293), hFc & Flag

CD40L/CD154/TRAP

CD40L (CD154; TRAP) is a ligand to CD40/TNFRSF5, acts function by generating a costimulatory signal that up-regulates IL-4 synthesis[1]. CD40L is specifically expressed on activated CD4+ T-lymphocytes and involves in activation of NF-κB/MAPK pathway[2][3]. CD40L also involves in B cell differentiation, maturation, and apoptosis[4]. CD40L in human, cleaved into 2 chains of membrane form (1-261 a.a.) and soluble form (113-261 a.a.) which serves as a ligand for integrins (ITGA5:ITGB1 and ITGAV:ITGB3). CD40L/CD154/TRAP Protein, Human (HEK293, hFc-Flag) has a total length of 248 amino acids (M13-L261), is expressed in HEK392 cells with N-terminal hFc- and Flag-tag.

SPRMSTBLIITCELISA细胞实验药物筛选

1.Measured by its binding ability in a functional ELISA. Immobilized CD40L at 2 μg/mL can bind Anti-CD40L Rabbit Monoclonal Antibody, the EC50 is ≤3.3 ng/mL. 2.Measured by its binding ability in a functional ELISA. Immobilized CD40 at 2 μg/mL can bind CD40L, the EC50 is 3.112-3.858 ng/mL. 3.Human CD40 protein hFc tag captured on COOH chip can bind Human CD40L protein hFc and Flag tag with an affinity constant < 2.06 nM as detected by LSPR Assay. 4. Immobilized Human CD40 Ligand Trimer, His Tag at 1 μg/mL (100 μl/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 1.29 μg/mL determined by ELISA. 5.Measured in a cell proliferation assay using human B cells (Ramos) in the presence of 10 ng/mL Human IL-4 (HY-P70445). The ED50 for this effect is 2.718 ng/mL, corresponding to a specific activity is 3.679×105 units/mg. Measured in a cell proliferation assay using human B cells (Ramos) in the presence of 10ng/mL Human IL-4 (HY-P70445). The ED50 for this effect is 2.718 ng/mL, corresponding to a specific activity is 3.679×105 units/mg.

CD40-L; T-cell antigen Gp39; NF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD154; sCD40L;

Human

HEK293

N-hFc;N-Flag

Greater than 90% as determined by SDS-PAGE.

P29965

M113-L261

Full Length of CD40L soluble form

44-50 kDa.

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

点位突变 标签定制 buffer定制 全长蛋白定制

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.