Analytical Data
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基因名
CD23/Fc epsilon RII
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简介
CD23/Fc epsilon RII Protein, a low-affinity receptor for IgE and CR2/CD21, regulates IgE production and B cell differentiation. On B cells, it initiates IgE-dependent antigen uptake and presentation to T cells. On macrophages, IgE binding induces intracellular killing of parasites through the L-Arginine-nitric oxide pathway. CD23/Fc epsilon RII homotrimer interacts with IGHE to regulate IgE homeostasis and with CR2/CD21 for binding. CD23/Fc epsilon RII Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD23/Fc epsilon RII protein, expressed by HEK293 , with N-His labeled tag.
- Application
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生物活性
Immobilized recombinant mouse CD23 at 5 μg/ml (100 μl/well) can bind Biotinylated Recombinant mouse IgE Protein. The ED50 for this effect is 0.1294 μg/mL. Immobilized recombinant mouse CD23 at 5 μg/ml (100 μl/well) can bind Biotinylated Recombinant mouse IgE Protein. The ED50 for this effect is 0.1294 μg/mL.
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别名
Low affinity immunoglobulin epsilon Fc receptor; BLAST-2; FCER2; CD23A
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种属
Mouse
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表达系统
HEK293
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P20693-1
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表达区间
E50-P331
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蛋白长度
Extracellular Domain
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分子量
33-43 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD23, also known as Fc epsilon RII, is a low-affinity receptor for immunoglobulin E (IgE) that plays a critical role in regulating immune responses, particularly in allergic reactions and asthma. This protein is predominantly expressed on the surface of B cells, dendritic cells, and other immune cells, serving as a key mediator in the interaction between the immune system and IgE, which is pivotal in allergy development and management. Research into CD23/Fc epsilon RII has gained significant attention due to its potential implications in understanding various allergic diseases and autoimmune disorders. Its dual role in promoting both the activation and regulation of B cell responses makes it a crucial target for therapeutic strategies aimed at modulating IgE-mediated hypersensitivity. Furthermore, the investigation of CD23's function and its interactions with other immune molecules could unlock novel avenues for immunotherapy, potentially leading to innovative treatments that alleviate allergic symptoms and enhance tolerance. The development of recombinant CD23 proteins facilitates detailed studies of its structure-function relationships, ligand interactions, and signaling pathways, paving the way for enhanced understanding of its biological significance and therapeutic potential in allergy and beyond.
