Analytical Data
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基因名
Fc epsilon RIA/FCER1A
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简介
The Fc epsilon RIA/FCER1A protein is a high-affinity receptor for IgE and critically mediates IgE effector function in myeloid cells. After binding to IgE and cross-linking with antigen, it activates signaling pathways that induce myeloid cell activation and differentiation. Fc epsilon RIA/FCER1A Protein, Human (His-SUMO) is the recombinant human-derived Fc epsilon RIA/FCER1A protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.
- Application
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生物活性
Measured by its binding ability in a functional ELISA.When Recombinant Human Fc epsilon RIA/FCER1A is immobilized at 0.1 μg/mL can bind Biotinylated Human IgE. The ED50 for this effect is 5.864 μg/mL. Measured by its binding ability in a functional ELISA.When Recombinant Human Fc epsilon RIA/FCER1A is immobilized at 0.1 μg/mL can bind Biotinylated Human IgE. The ED50 for this effect is 5.864 μg/mL.
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别名
Fc epsilon RI alpha; FCE1A; FCER1A; FcERI; FceRIa
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种属
Human
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表达系统
E. coli
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标签
N-6*His;N-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P12319
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表达区间
V26-Q205
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蛋白长度
Extracellular Domain
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分子量
37 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Fc epsilon receptor I alpha (FcεRIα) is a crucial protein involved in IgE-mediated allergic responses and is primarily found on the surface of mast cells and basophils. The engagement of IgE with FcεRIα leads to cell activation and the release of histamines and other mediators contributing to allergic diseases such as asthma, allergic rhinitis, and anaphylaxis. Understanding the structure and function of FcεRIα is essential for developing novel therapeutic strategies to manage allergic conditions. Recombinant FcεRIα protein serves as a valuable tool for investigating the molecular mechanisms underlying IgE signaling and receptor activation. It also facilitates the study of FcεRIα interactions with various ligands and monoclonal antibodies, which may lead to the identification of potential therapeutic inhibitors or modulators of allergic responses. Moreover, the characterization of recombinant FcεRIα contributes to the design of specific immunoassays for diagnosing allergy-related disorders. Overall, research on FcεRIα and its recombinant forms is pivotal for advancing our knowledge of allergic reactions and improving clinical approaches to allergy management.
