Analytical Data
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Gene name
CD64
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简介
CD64 Protein, with leukotriene receptor binding, crucially regulates processes like phagocytosis and pain perception, responding to epinephrine. Located in membrane rafts, CD64, encoded by FCGR1A, shows biased expression, especially in Spleen (RPKM 177.3) and Lung (RPKM 93.7). This emphasizes its importance in immune responses and sensory functions across diverse physiological contexts. CD64 Protein, Rat (HEK293, His) is the recombinant rat-derived CD64 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized Rat CD64-his at 10 μg/mL (100 μL/well) can bind biotinylated human IgG1. The ED50 for this effect is 0.0754 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized Rat CD64-his at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1,The ED50 for this effect is 0.0754 μg/mL.
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Alternative Names
High affinity immunoglobulin gamma Fc receptor I; Fcgr1; FcRI; CD64
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Species
Rat
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Source
HEK293
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
NP_001094306
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Expression Region
V17-P285
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AA Sequence
VVNATKAVVTLQPPWLSIFQEENVTLLCEGPHLGDSSTKWFINSTAIQISTPTYSILKASFKDSGEYKCQTGLSMPSDPVQLEIYRDWLLLQTSHRVLTEGEPLALRCHPWQNKKVYNVAFYRNGESLDFSQGSEVTILKTNLSHSGIYHCSAMGKKLFKSAGVSVTVKELFATPVLRVSLSSPFPEGSLVILNCETRLFLQSPGLRLYFSFYVGSKILEDRSTSSEYHIPRAEREDDGSYWCEVATEDGRVLKRSTKLELFGPQSSDP
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Protein Length
Partial
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Molecular Weight
40-42 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD64, also known as FcγRI, is a high-affinity receptor for the Fc portion of immunoglobulin G (IgG) and plays a critical role in the immune response by mediating phagocytosis and activating immune cells. Research on recombinant CD64 proteins has gained significant attention due to their potential applications in both therapeutic and diagnostic fields. The ability to produce CD64 in a recombinant form allows for the detailed study of its structure, function, and interactions with other immune molecules. Furthermore, recombinant CD64 has been explored as a target for antibody-drug conjugates and as a tool for enhancing the efficacy of antibody therapies, particularly in treating cancers and autoimmune diseases. Additionally, the study of CD64 expression on monocytes and macrophages serves as a valuable biomarker for understanding various pathological conditions, including infections, inflammatory diseases, and malignancies. Overall, the investigation of CD64 recombinant proteins contributes to elucidating immune mechanisms and developing novel therapeutic strategies aimed at improving patient outcomes.
