Analytical Data
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Gene name
CD133/PROM1
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简介
The CD133/PROM1 protein is critical for multiple processes including glomerular epithelial cell differentiation and retinal morphogenesis, showing biased expression in tissues such as lung and kidney. CD133/PROM1 Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD133/PROM1 protein, expressed by HEK293 , with N-mFc labeled tag.
- Application
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Alternative Names
Prominin-1; Antigen AC133; CD133; PROM1; PROML1; MSTP061
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Species
Rat
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Source
HEK293
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Tag
N-mFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
NP_001103607.1
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Expression Region
N171-Y424
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AA Sequence
NQQTRTRIQRTQKLAESNYRDLRALLTEAPKQIDYILGQYNTTKNKAFSDLDSIDSVLGGRIKGQLKPKVTPVLEEIKAMATAIRQTKDALQNMSSSLKSLRDASTQLSTNLTSVRNSIENSLNSNDCASDPASKICDSLRPQLSNLGSNHNGSQLQSVDRELNTVNDVDRTDLESLVKRGYMSIDEIPNMIQNQTGDVIKDVKKTLDSVSSKVKNMSQSIPVEEVLLQFSHYLNDSNRYIHESLPRVEEYDSY
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Protein Length
Partial
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Molecular Weight
70-95 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD133, also known as PROM1, is a transmembrane glycoprotein prominently expressed in stem cells and certain types of cancer cells, making it a critical marker in cancer research and regenerative medicine. Its role in maintaining stem cell properties and regulating cell differentiation has piqued the interest of scientists aiming to understand its involvement in tumorigenesis and tissue regeneration. The presence of CD133+ cells in various tumors, such as glioblastoma and colorectal cancer, indicates its potential as a target for cancer therapies, particularly in the context of cancer stem cells that contribute to tumor recurrence and metastasis. Recombinant CD133/PROM1 proteins have been developed to facilitate the study of its biological functions and interactions at the molecular level, allowing researchers to elucidate signaling pathways and mechanisms of action. Additionally, these proteins can serve as valuable tools for developing diagnostic and therapeutic strategies, including the creation of targeted drugs and vaccines. Overall, the ongoing research on CD133/PROM1 recombinant proteins holds promise for advancing our understanding of stem cell biology and improving clinical outcomes in cancer treatment.
