Analytical Data
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Gene name
ICOS
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简介
ICOS proteins optimize T cell responses to foreign antigens, promoting proliferation, lymphokine secretion, upregulation of cell-cell interaction molecules, and efficient B cell antibody secretion. ICOS is essential for efficient communication of T cells and B cells and for normal antibody responses to T cell-dependent antigens. ICOS Protein, Mouse (C137S, C138S, HEK293, Fc) is the recombinant mouse-derived ICOS protein, expressed by HEK293 , with C-hFc labeled tag and C137S, C138S mutation.
- Application
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Biological Activity
1. Immobilized Human B7-H2 His at 1 μg/mL (100 μL/well) can bind Mouse ICOS (C137S C138S, Fc) with a linear range of 0.3-10 ng/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse ICOS at 1 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human rhB7-H2. The ED50 for this effect is 37.18 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse ICOS at 1 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human rhB7-H2 .The ED50 for this effect is 37.18 ng/mL.
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Alternative Names
ICOS; CD278; AILIM; Inducible T-cell costimulator
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Species
Mouse
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9WVS0
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Expression Region
E21-L144, C137S, C138S
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Protein Length
Extracellular Domain
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Molecular Weight
40-52 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ICOS (Inducible T-cell COStimulator) is a member of the CD28 family of costimulatory molecules pivotal for T-cell activation and immune response regulation. Its role in mediating signals that promote T-cell proliferation, differentiation, and survival has made it a significant focus in immunological research. The ICOS pathway has been implicated in various diseases, particularly autoimmune disorders and cancers, as dysregulation of ICOS signaling can lead to excessive immune responses or inadequate tumor immunity. Consequently, researchers have been exploring ICOS as a therapeutic target. The study of recombinant ICOS proteins involves understanding their structure, function, and interactions with other immune molecules, enabling insights into their pivotal role in immune modulation. Additionally, recombinant ICOS proteins can be used in preclinical models to assess their potential as predictive biomarkers or therapeutic agents. The development of ICOS-targeting therapies, such as monoclonal antibodies or agonists, holds promise for enhancing immune responses in cancer immunotherapy or restoring balance in autoimmune diseases. Therefore, investigating ICOS recombinant proteins is crucial for developing innovative immunotherapeutic strategies that leverage the body's immune system to achieve more effective disease management.
