Recombinant Mouse GITR Protein(HEK293), hFc & His

GITR

GITR (TNFRSF18) is a member of the TNFR superfamily. GITR promotes T cell activation and proliferation and increases resistance to tumors and viral infections, and exacerbates autoimmune diseases and inflammation processes[1]. GITR Protein, Mouse (HEK293, Fc-His) is a recombinant protein with a C-terminal Fc label and a C-terminal His label, It consists of 132 amino acids (S22-H153) and is produced in HEK293 cells.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

Measured by its binding ability in a functional ELISA. When Recombinant Mouse GITR is immobilized at 2 µg/mL(100 µL/well) can bind Mouse GITR Ligand. The ED50 for this effect is 27.22 ng/mL. Measured by its binding ability in a functional ELISA. When Recombinant Mouse GITR is immobilized at 2 µg/mL (100 µL/well) can bind Mouse GITR Ligand. The ED50 for this effect is 27.22 ng/mL.

Tumor necrosis factor receptor superfamily member 18; CD357; Tnfrsf18; GITR

Mouse

HEK293

C-hFc;C-6*His

Greater than 90% as determined by SDS-PAGE.

O35714-1

S22-H153

Extracellular Domain

56 kDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.