Recombinant Human 4-1BB/TNFRSF9 Protein(HEK293), His

4-1BB/TNFRSF9

4-1BB (CD137; TNFRSF9), a receptor of TNFSF9/4-1BBL, belongs to the tumor necrosis factor (TNF) receptor superfamily[1]. 4-1BB is helpful for T cell activation and development, and also induces peripheral mononuclear cell proliferation and migration to the tumor microenvironment[2]. 4-1BB is also involved in enhancing Nrf2 and NF-κB pathway mediated apoptosis of endothelial cells[3]. Human 4-1BB protein is a surface glycoprotein with a transmembrane domain (187-213 a.a.). 4-1BB/TNFRSF9 Protein, Human (HEK293, His) is the extracellular part (L24-Q186) of 4-1BB protein, produced by HEK293 cells with C-terminal 6*His-tag.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

1.Loaded Human 4-1BB-His on HIS1K Biosensor, can bind Anti-Human CD137 mAb-Fc with an affinity constant of 25.5 nM as determined in BLI assay. 2.Immobilized Human 4-1BB-His at 10 μg/mL (100 μl/well) can bind Human 4-1BBL-Fc.The ED50 of Human IL-23-His is 1.61 μg/mL. 3. Loaded Tecaginlimab (HY-P99572) on AHC2 biosensor, can bind 4-1BB/TNFRSF9 Protein, Human (HEK293, His) with an affinity constant of 5.270E-11 M as determined in BLI assay. Loaded Tecaginlimab (HY-P99572) on AHC2 biosensor, can bind 4-1BB/TNFRSF9 Protein, Human (HEK293, His) with an affinity constant of 5.270E-11 M as determined in BLI assay.

CD137; ILA; TNFRSF9; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA

Human

HEK293

C-6*His

Greater than 90% as determined by SDS-PAGE.

Q07011

L24-Q186

Extracellular Domain

28-35 kDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.