Analytical Data
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Gene name
IL-10
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简介
IL-10 Protein, a key immune regulator, mitigates inflammation by binding to its receptor (IL10RA/IL10RB), activating JAK1 and STAT2, leading to STAT3-mediated anti-inflammatory gene expression. IL-10 targets APCs, reducing pro-inflammatory cytokines and hindering antigen presentation. It controls macrophage inflammation through metabolic reprogramming. Existing as a homodimer, IL-10 interacts with IL10RA/IL10RB. Animal-Free IL-10 Protein, Pig (His) is the recombinant pig-derived animal-FreeIL-10 protein, expressed by E. coli , with C-His labeled tag. This product is for cell culture use only.
- Application
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Alternative Names
Interleukin-10; IL10; IL-10; Cytokine synthesis inhibitory factor; CSIF
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Species
Pig
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Source
E. coli
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Tag
C-6*His
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Purity
> 90% as determined by SDS-PAGE.
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Uniprot
Q29055
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Expression Region
S19-N175
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Protein Length
Full Length of Mature Protein
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Molecular Weight
17-25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Interleukin-10 (IL-10) is a cytokine with potent anti-inflammatory properties, playing a crucial role in modulating immune responses. Its primary function involves inhibiting the production of pro-inflammatory cytokines and promoting the differentiation of regulatory T cells. Given its therapeutic potential, research into IL-10 recombinant protein has gained significant attention, particularly concerning its application in autoimmune diseases, chronic inflammatory conditions, and various cancers. The ability of IL-10 to balance immune activity and suppress excessive inflammation makes it an attractive candidate for novel therapeutic strategies. Studies have shown that recombinant IL-10 can enhance wound healing, support tissue regeneration, and mitigate the adverse effects of inflammatory diseases. Furthermore, its potential role in immunotherapy, particularly in enhancing anti-tumor immunity, is being actively explored. Preclinical and clinical trials have demonstrated encouraging results, suggesting that IL-10 administration can lead to improved patient outcomes. However, challenges remain in optimizing dosing strategies, delivery methods, and understanding the broader implications of IL-10 interactions within the immune microenvironment. As researchers continue to unravel the complex mechanisms behind IL-10's actions, the development of IL-10-based therapies holds promise for a range of medical applications, paving the way for innovative treatment modalities in managing immune-related disorders.
