Analytical Data
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Gene name
CD28
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简介
CD28 Protein plays a pivotal role in T-cell activation, promoting cell proliferation, cytokine production, and T-cell survival. Upon ligation with TCR/CD3 and CD40L costimulation, CD28 enhances the production of IL4 and IL10 in T-cells, contributing to immune response modulation. Additionally, isoform 3 of CD28 facilitates CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. The protein forms homodimers through disulfide linkages and interacts with various molecules. CD28 Protein, Canine (HEK293, His) is the recombinant canine-derived CD28 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Alternative Names
CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290
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Species
Canine
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9GKP3
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Expression Region
T18-P153
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Protein Length
Partial
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Molecular Weight
40-60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD28 is a vital co-stimulatory molecule expressed on T cells, playing a crucial role in their activation and function. It binds to its ligands, CD80 and CD86, present on antigen-presenting cells, providing essential signals for T cell proliferation, survival, and differentiation. Research into CD28 has advanced significantly, particularly concerning its role in immune responses, T cell activation pathways, and potential therapeutic applications in immunotherapy. Recombinant CD28 proteins have emerged as valuable tools for studying T cell biology, facilitating the understanding of T cell receptor signaling and co-stimulation. Through the production of recombinant CD28, researchers can investigate its interactions in a controlled environment, providing insights into its structural and functional properties. Moreover, understanding CD28's mechanisms may lead to novel strategies for enhancing anti-tumor immunity or regulating autoimmune diseases. The potential for using recombinant CD28 in combination therapies, alongside checkpoint inhibitors or other immunomodulatory agents, highlights its relevance in improving therapeutic outcomes in various diseases. Overall, the study of CD28 and its recombinant forms is essential for advancing our knowledge about immune regulation and developing next-generation immunotherapies.
