Analytical Data
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Gene name
CD63
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简介
The CD63 protein serves as a cell surface receptor for TIMP1 and is critical for activating signaling cascades. It contributes to ITGB1 and integrin signaling, activating AKT, FAK/PTK2 and MAP kinases to promote cell survival, cytoskeletal reorganization, adhesion, spreading and migration. CD63 Protein, Pig (HEK293, Fc) is the recombinant pig-derived CD63 protein, expressed by HEK293 , with N-hFc labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized Human BST2 at 2.5 μg/mL (100μL/well) can bind Pig CD63. The ED50 for this effect is 2.754 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized Human BST2 at 2.5 μg/mL (100μL/well) can bind Pig CD63. The ED50 for this effect is 2.754 μg/mL.
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Alternative Names
CD63 antigen; LAMP-3; Tspan-30; CD63; MLA1; TSPAN30
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Species
Pig
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Source
HEK293
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Tag
N-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
XP_003355519.1
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Expression Region
R108-F204
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Protein Length
Partial
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Molecular Weight
50 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD63 is a member of the tetraspanin family, which are integral membrane proteins that play crucial roles in cellular processes such as adhesion, migration, and signal transduction. As a prominent marker of exosomes and other extracellular vesicles, CD63 is implicated in various biological functions and disease states, notably in cancer progression and immune response. Research has highlighted its involvement in the formation of specialized microdomains in the plasma membrane, influencing cellular interactions and communication. Furthermore, CD63 has gained attention as a potential biomarker for identifying and characterizing exosomes derived from various cell types, including tumor cells, making it significant for diagnostic and therapeutic applications. The production of recombinant CD63 protein has become essential for elucidating its role in exosomal biology and for developing targeted therapies. Advances in protein engineering techniques have facilitated the generation of recombinant CD63, allowing for detailed studies into its structure, function, and interactions with other molecules. This knowledge is vital for understanding its mechanism in health and disease and for leveraging its properties in the development of innovative therapeutic strategies, such as drug delivery systems and cancer immunotherapies, that exploit the unique characteristics of exosomes displaying CD63. As research continues, elucidating the precise roles and mechanisms of CD63 could lead to significant breakthroughs in diagnostics and targeted treatments for various diseases, positioning it as a key player in innovative biotherapeutics.
