Analytical Data
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Gene name
CD42c/GP1B
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简介
The CD42c protein is a platelet surface membrane protein that actively assembles plugs by binding to von Willebrand factor (vWF) in the subendothelium. The GP-Ib heterodimer is formed from the disulfide-linked GP-Ib β subunit and one GP-Ib α subunit, which constitute this protein complex. CD42c/GP1BB Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD42c protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Alternative Names
Platelet glycoprotein Ib beta chain; GP-Ib beta; GP1BB
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Species
Rat
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9JJM7
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Expression Region
P27-C147
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Protein Length
Extracellular Domain
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Molecular Weight
45-55 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD42c, also known as glycoprotein Ib (GP1B), is a crucial component of the von Willebrand factor (vWF) receptor complex on the surface of platelets. This complex plays a significant role in hemostasis, as it mediates the initial adherence of platelets to sites of vascular injury, thereby facilitating the formation of a primary hemostatic plug. The GP1B subunit is composed of several domains, including an extracellular domain responsible for binding vWF, a transmembrane domain, and a cytoplasmic domain that interacts with signaling molecules. Research into the recombinant expression of CD42c/GP1B proteins has gained importance due to the potential for therapeutic applications in treating bleeding disorders, such as Glanzmann thrombasthenia and Bernard-Soulier syndrome. Furthermore, understanding the structural and functional properties of CD42c/GP1B can provide insights into platelet biology and the mechanisms underlying thrombosis and hemostatic disorders. Recombinant protein studies enable detailed investigations into the protein's binding affinities, signaling pathways, and interactions with other platelet receptors, paving the way for innovative treatments and diagnostic tools. Overall, the study of CD42c/GP1B recombinant proteins is vital for advancing our comprehension of hemostasis and developing novel strategies to manage blood-related diseases.
