Analytical Data
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Gene name
CD1A-B2M Heterodimer
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简介
CD1A-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived CD1A-B2M Heterodimer protein, expressed by HEK293, with C-His labeled tag., has molecular weight of ~45.2 (33.6+11.6) KDa.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized Rhesus Macaque CD1A-B2M Heterodimer, at 2μg/mL (100μL/well) can bind Anti-CD1A antibody. The ED50 for this effect is 0.1894 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized Rhesus Macaque CD1A-B2M Heterodimer, at 2μg/mL (100μL/well) can bind Anti-CD1A antibody. The ED50 for this effect is 0.1894 μg/mL.
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Alternative Names
T-cell surface glycoprotein CD1a; CD1A; Beta-2-microglobulin; B2M
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Species
Rhesus Macaque
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
NP_001139290 (D19-S299)&NP_001040602
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Expression Region
NP_001139290 (D19-S299)&NP_001040602 (I21-M119)
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Molecular Weight
33-50 & 12 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The CD1A-B2M heterodimer is a significant component in the immune system, playing a crucial role in the presentation of lipid antigens to T cells. CD1A, a member of the CD1 family of glycoproteins, is primarily expressed on the surface of antigen-presenting cells, including dendritic cells and thymocytes. It is responsible for binding and presenting lipid-based antigens, which are essential for the activation of specific subsets of immune cells, particularly the NKT (Natural Killer T) cells. The association with beta-2-microglobulin (B2M) is critical for the stability and surface expression of the CD1A molecule. Understanding the structure and function of the CD1A-B2M heterodimer can provide insights into various immunological processes and diseases, including autoimmunity, infection, and cancer. Recent advancements in recombinant protein technology have allowed researchers to produce purified CD1A-B2M heterodimers, facilitating studies on their antigen presentation mechanisms and potential therapeutic applications. Investigating this heterodimer is vital for developing novel immunotherapies and vaccines that harness the immune system's capacity to recognize and respond to lipid antigens, thus enhancing our ability to combat infectious diseases and tumors. This research area continues to grow, as elucidating the diverse roles of CD1A-B2M can lead to groundbreaking insights in immunology and therapeutic innovations.
