Analytical Data
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Gene name
BAFF/TNFSF13B
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简介
BAFF/TNFSF13B protein is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA, forming a key ligand-receptor pathway together with TNFSF13/APRIL. These interactions play a crucial role in stimulating B-cell and T-cell function and regulating humoral immunity. BAFF/TNFSF13B Trimer Protein, Human (HEK293, His-Flag) is the recombinant human-derived BAFF/TNFSF13B protein, expressed by HEK293 , with N-His, N-Flag labeled tag.
- Application
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Biological Activity
1. Immobilized Human BAFF Trimer, His Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Human BAFFR, hFc Tag with the EC50 of ≤0.14 μg/mL determined by ELISA. 2. Immobilized Human BAFF Trimer, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of ≤8 ng/mL determined by ELISA. 3. Immobilized Human BAFF Trimer, His Tag at 2 μg/mL (100 μl/well) on the plate. Dose response curve for Human BAFFR, hFc Tag with the EC50 of ≤ 60 ng/mL determined by ELISA.
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Alternative Names
BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
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Species
Human
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Source
HEK293
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Tag
N-8*His;N-Flag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y275-1
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Expression Region
T141-L285
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Protein Length
Partial
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Molecular Weight
53-60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BAFF (B-cell activating factor), also known as TNFSF13B, is a critical cytokine belonging to the tumor necrosis factor (TNF) superfamily that plays a vital role in B-cell development, activation, and survival. Dysregulation of BAFF levels has been implicated in various autoimmune diseases, such as systemic lupus erythematosus (SLE) and rheumatoid arthritis, as well as in certain B-cell malignancies. Understanding the structure and function of BAFF is essential for developing targeted therapies aimed at modulating immune responses. Research on recombinant BAFF proteins has gained momentum as these proteins can facilitate the investigation of BAFF's biological activities and mechanisms of action. Additionally, recombinant BAFF can be used as a tool for in vitro assays to explore B-cell signaling pathways and as a potential therapeutic agent to enhance or inhibit B-cell responses in various clinical settings. The generation of well-characterized recombinant BAFF proteins is pivotal for elucidating the cytokine's role in the immune system and for advancing novel treatments for B-cell-related disorders. Recent studies have focused on the production, purification, and functional characterization of these proteins, paving the way for better understanding of their implications in health and disease.
