Analytical Data
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Gene name
CD34
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简介
CD34 Protein is a transmembrane phosphoglycoprotein that exists on the surface of human and various animal cells. CD34 Protein is used as a cellular marker for hematopoietic stem cell sorting and as an islet endocrine cell marker. CD34 Protein plays an important role in cell adhesion, inflammatory cell chemotaxis, cell proliferation and differentiation, and enhancement of inflammatory response. CD34 Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD34 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized Rat CD34 at 2 μg/mL can bind Anti-CD34 antibody, the ED50 is 0.0279 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized Rat CD34 at 2 μg/mL can bind Anti-CD34 antibody, the ED50 is 0.0279 μg/mL.
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Alternative Names
Hematopoietic progenitor cell antigen CD34; CD34; HPCA1
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Species
Rat
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
B1PLB1
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Expression Region
E37-T291
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AA Sequence
ENSTQGVFPPVPTNESTEESITSSIPGSTSHYYLIGQDSNKTTPAISETTVNFTATPGTPSGSGTPHTFSQPQTYPTVTLPTTSDSTSTPEVEHSTWNTSLPSVNVSDYSPNNVSSEMISTTEPCAHTSSSVPSIIKGDIKCSGIRGVPFTQGICLELSEASSCEEFKKERGEELMQILCKKEETEADAGTSVCSLNLAQSEVRPECLLMVLANSTELSSKLQLMEKHQSDLRKLGIQSFNKQDLGSHQSYSRKT
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Protein Length
Partial
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Molecular Weight
95-130 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD37 is a tetraspanin protein that plays a crucial role in the immune system, particularly in the regulation of B cell activation and differentiation. It is predominantly expressed on various immune cells, including B cells, monocytes, and macrophages. Dysregulation or aberrant expression of CD37 has been implicated in several hematological malignancies, including lymphomas and leukemias, making it a potential target for therapeutic interventions. Researchers have focused on the production of recombinant CD37 protein to elucidate its structural and functional properties. This recombinant protein allows for detailed studies of CD37's role in immune signaling pathways and its interactions with other surface proteins. Additionally, the generation of CD37-targeted therapies, such as monoclonal antibodies or CAR T-cell approaches, holds promise for the treatment of CD37-expressing tumors. The ongoing investigation into CD37 not only enhances our understanding of immune regulation but also paves the way for novel immunotherapeutic strategies aimed at improving patient outcomes in CD37-associated malignancies.
