Analytical Data
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Gene name
IL-1R2
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简介
IL-1R2 is a competitive endogenous inhibitor of IL-1 signaling. IL-1R2 serves as a decoy receptor and competes with IL-1R1 for IL-1, it can also form a complex with IL-1RAP once it binds IL-1. IL-1R2 is implicated in various IL-1-mediated inflammatory diseases[1]. IL-1R2 Protein, Human (HEK293, His) is a recombinant human IL-1R2 protein with a His tag at the C-terminus and is expressed in HEK293 cells. It consists of 330 amino acids (F14-E346).
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized Rat IL-1R2 at 2 μg/mL (100 μL/well) can bind biotinylated Human IL-1 beta. The ED50 for this effect is 19.20 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Rat IL-1R2 at 2 μg/ml (100 μL/well) can bind biotinylated Human IL-1 beta .The ED50 for this effect is 19.20 ng/mL.
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Alternative Names
Interleukin-1 receptor type 2; IL-1R-2; CDw121b; IL-1R-beta; CD121b; mIL-1R2; sIL-1R2
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Species
Rat
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 95% as determined by reducing SDS-PAGE. as determined by SDS-PAGE.
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Uniprot
P43303
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Expression Region
Phe14~Ala355
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AA Sequence
FTTPAVVHTGRVSESPVTSEKHPVLGDDCWFRGRDFKSELRLEGEPVVLRCPLVPHSDTSSSSRSLLTWSKSDSSQLIPGDEPRMWVKDDTLWVLPAVQQDSGTYICTFRNASHCEQMSLELKVFKNTEASFPLVSYLQISALSSTGLLVCPDLKEFISSRTDGKIQWYKGSILLDKGNKKFLSAGDPTRLLISNTSMGDAGYYRCVMTFTYEGKEYNITRNIELRVKGITTEPIPVIISPLETIPASLGSRLIVPCKVFLGTGTSSNTIVWWMANSTFISVAYPRGRVTEGLHHQYSENDENYVEVSLIFDPVTKEDLNTDFKCVATNPRSFQSLHTTVKE
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Protein Length
Extracellular Domain
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Molecular Weight
50-60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
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Protein Description
Interleukin-1 receptor type 2 (IL-1R2) is a crucial regulator of the inflammatory response, acting primarily as a decoy receptor for interleukin-1 (IL-1) cytokines, including IL-1α and IL-1β. Unlike its signaling counterpart, IL-1R1, which propagates pro-inflammatory signals, IL-1R2 binds IL-1 with high affinity without activating downstream signaling pathways. This function positions IL-1R2 as a significant player in modulating immune responses and limiting excessive inflammation. Research has indicated that dysregulation of IL-1R2 may be implicated in various inflammatory diseases, autoimmune disorders, and certain cancers. Understanding the molecular mechanisms underlying IL-1R2 function could offer vital insights into therapeutic interventions aimed at controlling inflammation. Recent studies have focused on the production and characterization of recombinant IL-1R2, exploring its potential applications in experimental models and clinical settings. The recombinant protein serves as a valuable tool for investigating IL-1 signaling pathways and may contribute to the development of new strategies for treating diseases characterized by chronic inflammation. Thus, the study of IL-1R2 and its recombinant forms holds promise for advancing both basic and translational research in immunology and therapeutic development.
