Analytical Data
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Gene name
ICAM-3/CD50
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简介
The ICAM-3/CD50 protein acts as a ligand for the leukocyte adhesion protein LFA-1 and integrin α-D/β-2, suggesting involvement in cell adhesion. ICAM-3 cooperates with integrin α-L/β-2 to facilitate phagocytosis of apoptotic neutrophils, suggesting a role in immune response regulation and apoptotic cell clearance. ICAM-3/CD50 Protein, Human (HEK293, His-Fc) is the recombinant human-derived ICAM-3/CD50 protein, expressed by HEK293 , with C-hFc, C-His labeled tag.
- Application
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Alternative Names
Intercellular adhesion molecule 3; ICAM-3; CDw50; ICAM-R; CD50
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Species
Human
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Source
HEK293
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Tag
C-hFc;C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P32942
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Expression Region
Q30-H485
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Protein Length
Extracellular Domain
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Molecular Weight
125-135 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ICAM-3 (Intercellular Adhesion Molecule 3) and CD50 (also known as the lymphocyte function-associated antigen 1 or LFA-1) are important molecules involved in immune cell interactions and adhesion processes. Research into ICAM-3/CD50 recombinant proteins has garnered interest due to their roles in facilitating leukocyte migration, activation, and communication within the immune system. ICAM-3 is predominantly expressed on hematopoietic cells, where it helps in the stabilization of immune synapses, while CD50 plays a key part in the binding of leukocytes to endothelial cells during inflammatory responses. The combination of these proteins has potential applications in understanding immune mechanisms underlying various diseases, including autoimmune disorders and cancer. Furthermore, the engineering of ICAM-3/CD50 recombinant proteins allows for the exploration of their functional properties, aiding in the development of therapeutic strategies aimed at modulating immune responses. This research opens avenues for novel interventions, potentially leading to improved treatments for chronic inflammatory diseases and enhanced vaccine responses by harnessing the adhesion and signaling properties of these molecules in the dynamic landscape of the immune system.
