Analytical Data
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Gene name
Nectin-1
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简介
Nectin-1 is critical in cell adhesion and synaptogenesis, promotes neurite outgrowth, and serves as a receptor for alphaherpesviruses, including HSV-1, HSV-2, and pseudorabies virus. It promotes viral entry and is involved in homodimerization, trans-heterodimerization with NECTIN3, and interactions with FGFR, AFDN, and HSV glycoprotein D. Nectin-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Nectin-1 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 is present at 1 μg/mL, can bind Recombinant Mouse Nectin-1. The ED50 for this effect is 151 ng/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 is present at 1 μg/mL, can bind Recombinant Mouse Nectin-1.The ED50 for this effect is 151 ng/mL.
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Alternative Names
Poliovirus Receptor-Related Protein 1; Herpes Virus Entry Mediator C; Herpesvirus Entry Mediator C; HveC; Herpesvirus Ig-Like Receptor; HIgR; Nectin-1; CD111; PVRL1; HVEC; PRR1
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Species
Mouse
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Source
HEK293
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9JKF6
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Expression Region
Q31-A354
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AA Sequence
QVVQVNDSMYGFIGTDVVLHCSFANPLPSVKITQVTWQKASNGSKQNMAIYNPTMGVSVLPPYEKRVEFLRPSFIDGTIRLSGLELEDEGMYICEFATFPTGNRESQLNLTVMAKPTNWIEGTRAVLRARKGQDDKVLVATCTSANGKPPSAVSWETRLKGEAEYQEIRNPNGTVTVISRYRLVPSREAHRQSLACIVNYHLDRFRESLTLNVQYEPEVTIEGFDGNWYLQRTDVKLTCKADANPPATEYHWTTLNGSLPKGVEAQNRTLFFRGPITYSLAGTYICEATNPIGTRSGQVEVNITEFPYTPTPEHGRRAGQMPTA
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Protein Length
Extracellular Domain
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Molecular Weight
50-75 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Nectin-1, a member of the immunoglobulin superfamily, is a crucial cell adhesion molecule known for its roles in cell-cell adhesion and epithelium integrity. It functions as a receptor for various pathogens, including herpes simplex virus (HSV), facilitating viral entry into host cells. Given its significance in viral pathogenesis and the immune response, Nectin-1 has become a target of interest in therapeutic research. Researchers aim to develop Nectin-1 recombinant proteins to understand its structure-function relationships, immunogenic properties, and potential as a biomarker for diseases. Furthermore, these recombinant proteins can serve as tools for studying cellular interactions and for developing vaccines against HSV and other pathogens. By elucidating Nectin-1's role in inflammation and immune regulation, scientists hope to harness this knowledge for innovative treatments in virology and immunology. The ongoing exploration of Nectin-1 recombinant proteins may yield promising insights into novel therapeutic strategies, highlighting their potential in addressing infectious diseases and improving human health.
