Analytical Data
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基因名
Semaphorin-4D/SEMA4D
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简介
Semaphorin-4D/SEMA4D protein, a receptor for PLXNB1 and PLXNB2, regulates cell-cell signaling. It promotes inhibitory synapse development via PLXNB1, modulates neurite arborization through RHOA activation, and enhances viability of B-cells. SEMA4D induces endothelial cell migration via PTK2B/PYK2, SRC, and PI3K-AKT pathway. It forms homodimers and interacts with PLXNB2 and PLXNB1. Semaphorin-4D/SEMA4D Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-His labeled tag.
- Application
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别名
SEMA4D; Semaphorin-4D; M-Sema G; Semaphorin-C-like 2; Sema J; CD100; Semacl2
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种属
Rhesus Macaque
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
EHH24221.1
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表达区间
M22-R734
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蛋白长度
Partial
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分子量
80.7 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Semaphorin-4D (SEMA4D), a member of the semaphorin family of proteins, plays a critical role in regulating cell signaling, tissue development, and immune responses. Originally identified for its involvement in neural development and axon guidance, recent studies have revealed its significance in various pathological conditions, including cancer, autoimmune diseases, and neurodegenerative disorders. SEMA4D exerts its effects primarily through binding to receptors such as Plexin-B1, leading to diverse cellular responses including cell migration, apoptosis, and immune modulation. The aberrant expression of SEMA4D has been linked to tumor progression and metastasis, positioning it as a potential biomarker and therapeutic target in oncology. The generation of recombinant SEMA4D proteins facilitates in-depth studies of its molecular mechanisms, interactions with other signaling pathways, and effects on immune cell behavior. Researchers are employing these recombinant proteins to explore novel therapeutic strategies that could manipulate SEMA4D signaling for potential clinical applications. Understanding the precise role of SEMA4D in various biological contexts could pave the way for innovative treatments for diseases where SEMA4D is implicated.
