Recombinant Canine 4-1BB/TNFRSF9 Protein(HEK293) , His

4-1BB/TNFRSF9

4-1BB (CD137; TNFRSF9), is a surface glycoprotein, a receptor of TNFSF9/4-1BBL, belongs to the tumor necrosis factor (TNF) receptor superfamily[1]. 4-1BB is helpful for T cell activation and development, and also induces peripheral mononuclear cell proliferation and migration to the tumor microenvironment[2]. 4-1BB is also involved in enhancing Nrf2 and NF-κB pathway mediated apoptosis of endothelial cells[3]. CD137/4-1BB Protein, Canine (HEK293, His) has 185 amino acids (M1-S185), produced by HEK293 cells with C-terminal His-tag.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

Immobilized Canine CD137 at 10 µg/mL (100 µL/well) can bind Biotinylated Recombinant Rat TNFSF9. The ED50 for this effect is 1.722 µg/mL. Immobilized Canine CD137 at 10 µg/mL (100 µL/well) can bind Biotinylated Recombinant Rat TNFSF9. The ED50 for this effect is 1.722 µg/mL.

CD137; ILA; TNFRSF9; 4-1BB ligand receptor; CDw137

Canine

HEK293

C-His

Greater than 95% as determined by reducing SDS-PAGE.

XP_850336.1

I24-S185

Partial

24-34 kDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.