Analytical Data
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Gene name
Fas/CD95
- Application
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Biological Activity
Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 100.3 ng/mL in the presence of 2 ng/mL Human Fas Ligand, corresponding to a specific activity is 9.970×103 units/mg. Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 100.3 ng/mL in the presence of 2 ng/mL Human Fas Ligand, corresponding to a specific activity is 9.970×103 units/mg.
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Alternative Names
Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; CD95; FAS; APT1; FAS1; TNFRSF6
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Species
Rat
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
Q63199
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Expression Region
Q22-K170
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Protein Length
Extracellular Domain
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Molecular Weight
27-38 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Fas, also known as CD95, is a crucial cell-surface receptor that plays a significant role in the regulation of apoptosis, or programmed cell death. It belongs to the tumor necrosis factor receptor (TNFR) superfamily and primarily mediates the extrinsic apoptosis pathway. The interaction of Fas with its ligand, FasL, initiates a cascade of events leading to the activation of caspases, which are essential for executing the apoptotic program. This mechanism is vital for maintaining immune homeostasis, eliminating damaged or harmful cells, and preventing autoimmune diseases. Dysregulation of the Fas/CD95 pathway has been associated with various pathological conditions, including cancer, where tumor cells often evade apoptosis, allowing for uncontrolled proliferation. Additionally, insufficient Fas signaling can lead to autoimmune disorders due to the accumulation of autoreactive lymphocytes. In this context, the study of Fas/CD95 recombinant proteins has gained significant attention, as they can serve as valuable tools for understanding the intricate mechanisms of apoptosis and its implications in health and disease. Researchers are particularly interested in developing therapeutic strategies that manipulate the Fas pathway, either by enhancing its function to induce apoptosis in cancer cells or by inhibiting excessive apoptosis in autoimmune conditions. Moreover, recombinant Fas/CD95 proteins can be used in assays to study receptor-ligand interactions, signaling pathways, and the effects of small molecules or antibodies, paving the way for the development of targeted therapies. Overall, the research on Fas/CD95 recombinant proteins holds promise for advancing our understanding of apoptosis and its diverse roles in immunology and oncology.
