Analytical Data
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基因名
OX40/TNFRSF4
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简介
OX40 (TNFRSF4), is a receptor for OX40 Ligand. OX40 is preferentially expressed by T cells. OX40 can be activated by OX40 Ligand, thereby functioning as a T cell co-stimulatory molecule. The OX40-OX40 Ligand interaction promotes effector T-cell survival and effectively induces memory T-cell generation, as well as enhances the helper function of Tfh for B cells, and also promotes the differentiation and maturation of DCs[1][2]. OX40/TNFRSF4 Protein, Human (HEK293, His) is a recombinant human OX40 (L29-A216) with C-terminal His tag, which is produced in HEK293.
- Application
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生物活性
1.2 µg/mL (100 µL/well) of immoblized recombinant human OX40/TNFRSF4-His can bind human Biotin-OX40L-His with a linear range of 1-20 ng/mL. 2.Immobilized Mouse OX40L-His at 10 μg/mL (100 μL/well) can bind Human OX40-6*His: Biotinylated by NHS-biotin prior to testing The ED50 of Recombinant Human OX40-6His is ≤9 μg/mL. 3. Loaded Telazorlimab (HY-P99570) on AHC2 biosensor, can bind OX40/TNFRSF4 Protein, Human (HEK293, His) with an affinity constant of 2.951E-08 M as determined in BLI assay. Loaded Telazorlimab (HY-P99570) on AHC2 biosensor, can bind OX40/TNFRSF4 Protein, Human (HEK293, His) with an affinity constant of 2.951E-08 M as determined in BLI assay.
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别名
rHuOX40/TNFRSF4, His; ACT35 antigen; CD134; TXGP1L
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种属
Human
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P43489
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表达区间
L29-A216
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氨基酸序列
LHCVGDTYPSNDRCCHECRPGNGMVSRCSRSQNTVCRPCGPGFYNDVVSSKPCKPCTWCNLRSGSERKQLCTATQDTVCRCRAGTQPLDSYKPGVDCAPCPPGHFSPGDNQACKPWTNCTLAGKHTLQPASNSSDAICEDRDPPATQPQETQGPPARPITVQPTEAWPRTSQGPSTRPVEVPGGRAVAHHHHHH
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蛋白长度
Partial
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分子量
38-45 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
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Protein Description
OX40, also known as TNFRSF4, is a member of the tumor necrosis factor receptor (TNFR) superfamily that plays a vital role in regulating immune responses, particularly in the activation and survival of T cells. Its interaction with the ligand OX40L enhances T cell proliferation, survival, and cytokine production, making it a critical co-stimulatory molecule in the immune system. Research has shown that OX40 signaling can improve anti-tumor immunity and has been implicated in various diseases, including autoimmune disorders and cancer. The development of OX40/TNFRSF4 recombinant proteins has become a focus of investigation, as these proteins can serve as therapeutic agents or tools for studying OX40 biology in detail. By understanding the mechanisms of OX40 engagement and its downstream signaling pathways, researchers aim to harness this knowledge for therapeutic interventions, including immunotherapies that could potentiate or restore T cell function in cancer and chronic infections. Exploring the structural and functional aspects of OX40/TNFRSF4 recombinant proteins will enhance our comprehension of their roles in immune regulation and could lead to novel strategies for enhancing anti-tumor immunity or modulating immune responses in autoimmune diseases.
