Analytical Data
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基因名
NKG2A
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简介
The NKG2A protein is an important immunosuppressive receptor that forms a complex with KLRD1 on lymphocyte subsets for self-non-self discrimination. It recognizes HLA-E loaded with self-peptides, monitors MHC class I expression in healthy cells, and promotes self-tolerance. NKG2A Protein, Cynomolgus/Rhesus Macaque (HEK293, Fc) is the recombinant Rhesus Macaque-derived NKG2A protein, expressed by HEK293 , with N-hFc labeled tag.
- Application
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生物活性
Measured by its binding ability in a functional ELISA. When Recombinant Human CD94 is present at 2 μg/mL, can bind Recombinant Cynomolgus NKG2A. The ED50 for this effect is 4.181 μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human CD94 is present at 2 μg/mL, can bind Recombinant Cynomolgus NKG2A. The ED50 for this effect is 4.181 μg/mL.
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别名
NKG2-A/NKG2-B type II integral membrane protein; CD159a; KLRC1
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种属
Rhesus Macaque
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表达系统
HEK293
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标签
N-hFc
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
Q9MZJ2
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表达区间
P94-L233
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分子量
50-55 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NKG2A is an inhibitory receptor primarily expressed on natural killer (NK) cells and some T cells, playing a crucial role in regulating immune responses. It interacts with its ligand, HLA-E, which presents peptides derived from major histocompatibility complex (MHC) class I molecules. This interaction is vital for maintaining immune tolerance and preventing autoimmunity. Research on NKG2A has gained traction due to its implications in cancer immunotherapy; tumors often exploit this pathway to evade immune detection by upregulating HLA-E expression, leading to decreased NK cell activity. Consequently, targeting NKG2A via monoclonal antibodies has emerged as a promising strategy in enhancing anti-tumor responses, making it a focal point in studies aimed at improving cancer treatment efficacy. Recent advancements in recombinant protein technology have facilitated the production of NKG2A-Fc fusion proteins, which can be used to block NKG2A’s inhibitory signals and restore NK cell function against malignancies. These innovations not only enhance our understanding of the immune evasion mechanisms employed by tumors but also pave the way for novel therapeutic interventions that can potentially improve patient outcomes in immuno-oncology.
