Analytical Data
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基因名
Aminopeptidase A
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简介
Aminopeptidase A, modulated by calcium, regulates central hypertension by preferentially cleaving N-terminal acidic residues from peptides, including angiotensin II. Aminopeptidase A Protein, Rat (sf9, His) is the recombinant rat-derived Aminopeptidase A protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
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别名
Glutamyl aminopeptidase; EAP; AP-A; CD249; ENPEP
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种属
Rat
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表达系统
Baculovirus
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P50123-1
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表达区间
R41-P945
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氨基酸序列
RSCEPGTTPAPSNPPPHTSTALPPQDQNVCPDSDDESGEWKNFRLPDFIQPVHYDLEVKVLMEEDRYTGIVSISVNLSKDTRDLWLHIRETRITKLPELRRPSGEQVPIRRCFEYKKQEYVVIQAEEDLAATSGDSVYRLTIEFEGWLNGSLVGFYRTTYTEDGQTKSIAATDHEPTDARKSFPCFDEPNKKATYNISLIHPKEYSALSNMPVEKKETLDNDWKKTTFMKSVPMSTYLVCFAVHQFTSIQRTSRSGKPLTVYVQPNQKQTAEYAANITKAVFDFFEDYFAMEYSLPKLDKIAIPDFGTGAMENWGLVTYRETNLLYDPLLSASSNQQRVASVVAHELVHQWFGNIVTMDWWDDLWLNEGFASFFEFLGVNHAEADWQMLSQVLLEDVLPVQEDDSLMSSHPVVVTVSTPAEITSVFDGISYSKGASILRMLQDWITPEKFQKGCQIYLENFKFKNAKTSDFWDSLEKASNQPVKEVMDTWTSQMGYPVVTVSGKQNVTQKRFLLDYKADPSQPPSALGYTWNIPIKWTENGNSNITVYYRSNREGITLNANLSGDGFLKINPDHIGFYRVNYEAETWDWIAETLSSNHMNFSSADRSSFIDDAFALARAQLLDYEKALNLTRYLTSEKDFLPWERVISAVSYIISMFEDDRELYPLIETYFRSQVKPIADSLGWQDTGSHITKLLRASVLGFACKMGAGEALGNASQLFEAWLKGNESIPVNLRLLVYRYGMQNSGNEAAWNYTLEQYQKTSLAQEKEKLLYGLASVKDVTLLARYLEMLKDPNIIKTQDVFTVIRYISYNSYGKSMAWNWIQLNWDYLVNRFTINDRYLGRIVTIAEPFNTELQLWQMQSFFAKYPNAGAGAKPREQVLETVKNNIEWLKLNRKSISEWFTSMP
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蛋白长度
Extracellular Domain
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分子量
116 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
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Protein Description
Aminopeptidase A (APA) is an important enzyme involved in the metabolism of neuropeptides and angiotensin, playing a crucial role in blood pressure regulation and neuronal signaling. Its primary function is to catalyze the cleavage of N-terminal amino acids from peptides, thus modulating bioactive peptide levels in various physiological processes. Research into recombinant APA has gained momentum due to its potential therapeutic implications, particularly in the context of hypertension and neurodegenerative diseases. The production of recombinant APA allows for a more detailed study of its enzymatic mechanisms, substrate specificity, and regulatory pathways. Additionally, achieving high yields of functional protein is essential for structural and functional analyses, facilitating insights into its catalytic activity and interactions with inhibitors. The development of robust expression systems has enabled scientists to produce APA in sufficient quantities for extensive characterizations, paving the way for the design of targeted inhibitors that could serve as novel therapeutic agents. Furthermore, understanding the molecular structure and dynamics of recombinant APA can provide valuable information for drug discovery efforts aimed at treating conditions associated with dysregulated peptide metabolism. Overall, the study of recombinant aminopeptidase A holds significant promise for advancing our understanding of its biological functions and potential applications in medicine.
