Analytical Data
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Gene name
BCAM
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简介
BCAM Protein, acting as a laminin alpha-5 receptor, potentially participates in intracellular signaling, indicated by its resemblance to related proteins. BCAM Protein, Mouse (HEK293, His) is the recombinant mouse-derived BCAM protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Measured by the ability of the immobilized protein to support the adhesion of HOS human osteogenic sarcoma cells. When 5x104 cells per well are added to Recombinant Mouse BCAM coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 0.491 μg/mL. Corresponding to a specific activity is 2.036×103 U/mg Measured by the ability of the immobilized protein to support the adhesion of HOS human osteogenic sarcoma cells. When 5 x 104 cells per well are added to Recombinant Mouse BCAM coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 0.491 μg/mL, corresponding to a specific activity is 2.036 x 103 U/mg
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Alternative Names
Basal cell adhesion molecule; B-CAM cell surface glycoprotein; CD239; Bcam; Gplu; Lu
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Species
Mouse
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Source
HEK293
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Tag
C-His
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
Q9R069
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Expression Region
E26-A541
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AA Sequence
ELHVSVPPRVEVMRGEQVALDCTPREHPEHYVLEWFLVDGTGARHRLASVEPQGSEFLGTVHSLGRVPPYEVDSRGRLVIAKVQVGDGRDYVCVVKAGAAGTSEATSSVRVFATPEDTEVSPNKGTLSVMDQFAQEIATCSSNNGNPVPRITWYRNGQRLEVPMEVNQKGYITIRTVREASGLYSLTSTLYLRLHKDDRDANFHCAAHYDLPSGQHGRLDSHTFRLTLHYPTEHVEFWVGSPSTTEGWVREGDAVQLLCQGDGSPSPEYSFFRQQGTQEEQLNVNLKGNLTLERVHRNQSGIYGCRVEDYDADEEVQLVKKLKLHVAYLDPLELSVPEELFVFLNSSSTVVNCSARGLPTPTVRWTKDSVTLADGPMLSLQSVTFDSAGTYTCEASTPTVPLLSRTQSFQLIVQGAPELKPNEIMPKSGNSWTEGDEVMLTCSARGFPEPKLTWSQRGDTPAEPPFEGRGWKSSSLMVKVTSALSREGVSCEASNIHGKKGHVFHFGSVAPQTAQA
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Protein Length
Extracellular Domain
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Molecular Weight
70-90 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
BCAM (Basal Cell Adhesion Molecule) is a cell adhesion molecule that plays a crucial role in various physiological and pathological processes, including cell migration, tissue development, and immune responses. It is primarily expressed in endothelial and epithelial tissues and is involved in maintaining tissue integrity and function. Research has shown that BCAM is implicated in several diseases, such as cancer, where its expression levels can influence tumor progression and metastasis. Given its significance, BCAM has gained attention as a potential biomarker and therapeutic target. Recent studies have focused on the recombinant expression of BCAM to better understand its structure-function relationships and to develop potential applications in targeted therapies. Through reconstitution of BCAM in various expression systems, researchers aim to produce functional proteins for further investigation into their interactions with other cellular components. This research could provide insights into the mechanisms by which BCAM contributes to disease processes, ultimately leading to the development of novel diagnostic and therapeutic strategies, particularly in oncology and inflammatory diseases. As the scientific community continues to unravel the complex roles of BCAM, the potential for exploiting its properties for medical advancements remains a promising area of study.
