Analytical Data
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Gene name
LAG-3
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简介
LAG-3 (lymphocyte activation gene 3) protein is expressed on antigen-activated T cells and transmits inhibitory signals by binding to ligands such as FGL1.FGL1 is the main ligand responsible for the suppressive function of LAG3 T cells.LAG-3 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived LAG-3 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Biological Activity
1. Measured by its ability to bind FGL1 Protein, Mouse, Recombinant (His Tag) in functional ELISA. 2. Immobilized Mouse LAG-3, hFc Tag at 5 μg/mL (100 μL/well) can bind Mouse FGL1 (HY-P75189). The ED50 for this effect is 0.1192 μg/mL, corresponding to a specific activity is 8.39×10^3 Unit/mg. Immobilized Mouse LAG-3, hFc Tag at 5 μg/mL (100 μL/well) can bind Mouse FGL1 (HY-P75189). The ED50 for this effect is 0.1192 μg/mL, corresponding to a specific activity is 8.39×103 Unit/mg.
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Alternative Names
Lymphocyte activation gene 3 protein; Protein FDC; CD223
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Species
Mouse
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
Q61790
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Expression Region
S23-L442
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AA Sequence
SGPGKELPVVWAQEGAPVHLPCSLKSPNLDPNFLRRGGVIWQHQPDSGQPTPIPALDLHQGMPSPRQPAPGRYTVLSVAPGGLRSGRQPLHPHVQLEERGLQRGDFSLWLRPALRTDAGEYHATVRLPNRALSCSLRLRVGQASMIASPSGVLKLSDWVLLNCSFSRPDRPVSVHWFQGQNRVPVYNSPRHFLAETFLLLPQVSPLDSGTWGCVLTYRDGFNVSITYNLKVLGLEPVAPLTVYAAEGSRVELPCHLPPGVGTPSLLIAKWTPPGGGPELPVAGKSGNFTLHLEAVGLAQAGTYTCSIHLQGQQLNATVTLAVITVTPKSFGLPGSRGKLLCEVTPASGKERFVWRPLNNLSRSCPGPVLEIQEARLLAERWQCQLYEGQRLLGATVYAAESSSGAHSARRISGDLKGGHL
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Protein Length
Partial
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Molecular Weight
82-96 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
LAG-3 (Lymphocyte Activation Gene-3) is an immune checkpoint receptor that plays a significant role in regulating T cell activity and maintaining immune homeostasis. It is highly expressed on activated T cells, regulatory T cells, and certain other immune cells. In cancer immunotherapy, LAG-3 has garnered considerable attention due to its ability to inhibit T cell function, making it a potential target for enhancing anti-tumor immunity. Research has shown that LAG-3 interacts with MHC class II molecules, leading to a downregulation of T cell activation and proliferation. Understanding the molecular mechanisms underlying LAG-3 signaling and its role in immune escape by tumors has become a critical focus. Additionally, the development of LAG-3-targeted therapies, including monoclonal antibodies and recombinant proteins, is being explored to improve the efficacy of existing immunotherapeutic approaches. These studies aim not only to block LAG-3’s inhibitory effects but also to use LAG-3 as a biomarker for patient stratification in immunotherapy. The exploration of LAG-3 recombinant proteins has attracted interest as tools for both studying its biology and advancing therapeutic strategies, particularly in combination with other immune modulators. Overall, ongoing research on LAG-3 continues to shed light on its dual role in immune regulation and its potential as a therapeutic target in various diseases, including cancer and autoimmune disorders.
