Analytical Data
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Gene name
TRAIL/TNFSF10
- Application
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Biological Activity
1.The ED50 is <40>2.5 × 104 units/mg. 2.Measured in a cytotoxicity assay using L929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 1.484 ng/mL, corresponding to a specific activity is 6.738×105 units/mg. Measured in a cytotoxicity assay using L929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 1.484 ng/mL, corresponding to a specific activity is 6.738×105 units/mg.
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Alternative Names
rHuTRAIL/Apo2L; TNFSF10; CD253
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P50591
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Expression Region
V114-G281
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Protein Length
Partial
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Molecular Weight
20 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TRAIL (TNF-Related Apoptosis-Inducing Ligand) is a member of the tumor necrosis factor (TNF) superfamily that plays a crucial role in regulating apoptosis, particularly in cancer therapy. It selectively induces programmed cell death in tumor cells while sparing normal cells, making it a promising candidate for cancer treatment. The TRAIL ligand interacts with five distinct receptors, namely TRAIL-R1 to TRAIL-R4 and decoy receptor DcR1/DcR2, triggering apoptosis through the extrinsic pathway when bound to its pro-apoptotic receptors. However, many cancer cells can develop resistance to TRAIL, limiting its therapeutic efficacy. Research into TRAIL/TNFSF10 recombinant proteins focuses on enhancing its effectiveness by exploring various strategies, including combination therapies, the development of TRAIL-based agonistic antibodies, and the use of engineered variants that improve its stability and bioavailability. This line of research aims to overcome resistance mechanisms and broaden the application of TRAIL in clinical oncology, potentially integrating it into multimodal treatment approaches. The ongoing investigation into the mechanistic underpinnings of TRAIL-induced apoptosis and the development of innovative delivery methods continues to be pivotal for advancing cancer immunotherapy and improving patient outcomes.
