Analytical Data
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Gene name
IL-15R alpha & IL-15
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简介
IL-15R alpha is a high affinity receptor for IL-15 (Kd: 100 pM)[1]. IL-15R alpha binds IL-15 and thereby activating the antitumor functions of NK cells and CD8+ T cells[2]. IL-15R alpha plays an important role in memory CD8+ T cell homeostasis and lymphocyte development[3]. IL-15R alpha Protein, Mouse (HEK293, hFc) is a recombinant mouse extracellular region of IL-15R alpha (G33-K205) with a C-Terminal hFc tag, which is produced in HEK293 cells.
- Application
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Biological Activity
Measured by its ability to block human IL-15-induced proliferation of CTLL-2 mouse cytotoxic T cells.The ED50 for this effect is <10 ng/mL. Measured by its ability to block human IL-15-induced proliferation of CTLL-2 mouse cytotoxic T cells. The IC50 for this effect is 0.4892 ng/mL in the presence of 10 ng/mL human IL-15 (HY-P7034).
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Alternative Names
Il15ra; Interleukin-15 receptor subunit alpha; IL-15 receptor subunit alpha; IL-15R-alpha; IL-15RA; CD antigen CD215; Soluble interleukin-15 receptor subunit alpha; sIL-15 receptor subunit alpha; sIL-15R-alpha; sIL-15RA;
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Species
Mouse
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by reducing SDS-PAGE.
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Uniprot
Q60819-1
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Expression Region
G33-K205
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Protein Length
Extracellular Domain
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Molecular Weight
46-90 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Interleukin-15 (IL-15) is a crucial cytokine involved in immune responses, particularly in the development, activation, and proliferation of natural killer (NK) cells and memory CD8+ T cells. The IL-15 receptor (IL-15R) is a complex system composed of three subunits: IL-15R alpha (IL-15Rα), IL-2R beta (IL-2Rβ), and common gamma chain (γc). Among these, IL-15Rα plays a significant role in the binding and presentation of IL-15 to its receptor complex, facilitating signaling pathways that promote cell survival and proliferation. Research on recombinant forms of IL-15, particularly those fused with IL-15Rα, has garnered attention due to their potential therapeutic applications in cancer immunotherapy and the treatment of immunodeficiencies. The combination of IL-15 with its receptor alpha enhances the stability, bioavailability, and specificity of IL-15, leading to improved immune responses. Studies have shown that recombinant IL-15 and IL-15Rα fusion proteins can enhance the proliferation and function of NK cells and CD8+ T cells, making them promising candidates for clinical applications. Understanding the molecular interactions of IL-15 and IL-15Rα is essential for optimizing these recombinant proteins and developing novel immunotherapeutic strategies. This area of research is crucial for advancing our knowledge of cytokine biology and harnessing the immune system to target various malignancies and infectious diseases effectively.
