Analytical Data
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Gene name
CD81
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简介
The CD81 protein has important effects on cellular processes and is involved in CD19 receptor trafficking on activated B cells. This facilitates CD19-CR2 and B cell receptor complex assembly, lowering the antigen dose threshold for B cell clonal expansion. CD81 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CD81 protein, expressed by HEK293 , with N-mFc labeled tag.
- Application
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Biological Activity
Immobilized Mouse CD81 at 1 μg/mL (100 μL/well) can bind Anti-CD81 Antibody. The ED50 for this effect is 0.741 μg/mL. Immobilized Mouse CD81 at 1 μg/mL (100 μL/well) can bind Anti-CD81 Antibody, The ED50 for this effect is 0.741 μg/mL.
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Alternative Names
CD81 antigen; CD81; CVID6; TAPA-1; TSPAN28
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Species
Mouse
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Source
HEK293
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Tag
N-mFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P35762
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Expression Region
K116-K201
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Protein Length
Partial
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Molecular Weight
36.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD81 is a tetraspanin protein widely recognized for its role in various cellular processes, including immune response, cell adhesion, and viral entry. It is a key player in the formation of tetraspanin-enriched microdomains on the cell surface, facilitating the organization of protein complexes that are crucial for signal transduction and cell communication. The research surrounding recombinant CD81 protein has gained traction due to its implications in understanding viral infections, particularly in the context of hepatitis C virus (HCV), which utilizes CD81 as a co-receptor for cell entry. Additionally, CD81's involvement in B cell activation and its potential as a therapeutic target for autoimmune disorders have sparked interest in its functional characterization. The production of recombinant CD81 allows for detailed studies of its structural properties, ligand interactions, and the mechanisms underlying its biological functions. This research is essential for developing strategies to manipulate CD81's activity in disease contexts and for exploring its role in other pathogens, potentially leading to novel antiviral therapies or immunotherapeutic approaches. Understanding the intricate dynamics of CD81 through recombinant technology can thus provide valuable insights into its biological significance and therapeutic potential across various fields of medicine.
