Analytical Data
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Gene name
IL-17A
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简介
The IL-17A protein has important heterodimerization and homodimerization activities and is involved in a variety of processes, including responses to glucocorticoid stimulation and the regulation of cell death and transcription. IL-17A is present in the cytoplasm and extracellular space and has been implicated in diseases such as pancreatitis, inflammatory bowel disease, renal disease, and periodontal disease, and may serve as a biomarker. IL-17A Protein, Rat is the recombinant rat-derived IL-17A protein, expressed by E. coli.
- Application
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Biological Activity
Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.7119 ng/mL, corresponding to a specific activity is 1.4×106 U/mg. Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells.The ED50 for this effect is 0.7119 ng/mL, corresponding to a specific activity is 1.4×106 U/mg.
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Alternative Names
CTLA8; CTLA-8; IL-17; Interleukin-17A; IL17A
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Species
Rat
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by reducing SDS-PAGE. as determined by SDS-PAGE.
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Uniprot
NP_001100367
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Expression Region
A26-S158
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Protein Length
Full Length of Mature Protein
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Molecular Weight
17 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Interleukin-17A (IL-17A) is a pro-inflammatory cytokine primarily produced by a subset of T cells known as Th17 cells. It plays a crucial role in immune responses against extracellular pathogens and is implicated in various autoimmune and inflammatory diseases, including rheumatoid arthritis, psoriasis, and multiple sclerosis. The significance of IL-17A in modulating inflammation has spurred extensive research into its biological functions and potential therapeutic applications. Recombinant IL-17A proteins have been developed to better understand its signaling pathways and interactions with other immune components. These proteins serve as valuable tools for exploring the mechanisms underlying IL-17A-related pathologies and for identifying novel therapeutic targets. Moreover, the investigation of IL-17A-recombinant proteins has paved the way for the development of monoclonal antibodies that inhibit IL-17A activity, which have shown promise in clinical settings. By blocking IL-17A signaling, these agents can effectively reduce inflammation and improve outcomes for patients suffering from autoimmune diseases. Continuing research on IL-17A and its recombinant forms is essential not only for elucidating its role in immune regulation but also for advancing therapeutic strategies aimed at mitigating the detrimental effects of excessive IL-17A activity in various inflammatory conditions.
