Analytical Data
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Gene name
CD98
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简介
The CD98 protein acts as a chaperone, promoting the biogenesis and trafficking of functional transporter heterodimers to the plasma membrane. It forms heterodimers with various SLC7 family transporters and affects the substrate specificity of amino acid antiporters. CD98 Protein, Human (HEK293, His) is the recombinant human-derived CD98 protein, expressed by HEK293 , with N-His labeled tag.
- Application
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Biological Activity
1.Immobilized CD98, Human His Tag at 0.5 μg/mL (100μl/well) on the plate. Dose response curve for Anti-CD98 Antibody, mFc Tag with the EC50 of 11.1 ng/mL determined by ELISA. 2.Immobilized Human CD98 at 2 μg/mL (100 μL/well) can bind Anti-CD98 Antibody. The ED50 for this effect is 0.03379 nM. Immobilized Human CD98 at 2 μg/mL (100 μL/well) can bind Anti-CD98 Antibody, The ED50 for this effect is 0.03379 nM.
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Alternative Names
4F2 cell-surface antigen heavy chain; 4F2hc; CD98; SLC3A2
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Species
Human
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Source
HEK293
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Tag
N-His
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P08195-1
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Expression Region
R206-A630
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AA Sequence
RAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA
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Protein Length
Extracellular Domain
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Molecular Weight
60-80 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD98 is a transmembrane protein that functions as an integrin-associated protein and is involved in multiple cellular processes, including cell adhesion, proliferation, and amino acid transport. Originally identified for its role in lymphocyte activation, CD98 has garnered significant interest in cancer research due to its overexpression in various malignant tumors, suggesting its potential as a biomarker and therapeutic target. The protein consists of several key domains that facilitate its interaction with integrins and other signaling molecules, thereby influencing cell signaling pathways that drive tumorigenesis. Recent studies have focused on the structural and functional characterization of CD98 recombinant protein to elucidate its role in cancer progression and metastasis. By generating and purifying CD98 recombinant protein, researchers aim to investigate its interactions with ligands, understand its physiological functions, and explore its potential as a target for novel anti-cancer therapies. Insights gained from these studies could lead to the development of strategies that inhibit CD98 function, ultimately aiming to halt tumor growth and improve patient outcomes. Overall, the exploration of CD98 recombinant protein serves as a critical step in advancing our understanding of its biology and therapeutic potential in oncology.
