Analytical Data
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基因名
TLR3
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简介
The TLR3 protein is a key Toll-like receptor family member that plays an important role in the innate immune system's recognition of pathogen-associated molecular patterns, especially viral double-stranded RNA. Its research enhances understanding of immune responses and provides potential applications for vaccine development and antiviral treatments. TLR3 Protein, Human (HEK293, His) is the recombinant human-derived TLR3 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
1.Immobilized Human TLR3 at 0.5 μg/mL (100μL/Well) on the plate. Dose response curve for Anti-TLR3 Antibody with the EC50 < 13.6 ng/mL determined by ELISA. 2.Measured by its ability to inhibit poly I:C induced IL-8 secretion by HEK293 human embryonic kidney cells transfected with TLR3. The ED50 for this effect is 4.109 µg/mL, corresponding to a specific activity is 243.368 U/mg in the presence of 0.2 µg/mL of poly I:C. Measured by its ability to inhibit poly I:C induced IL-8 secretion by HEK293 human embryonic kidney cells transfected with TLR3. The ED50 for this effect is 4.109 µg/mL, corresponding to a specific activity is 243.368 U/mg in the presence of 0.2 µg/mL of poly I:C.
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别名
TLR3; CD283; IIAE2
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种属
Human
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
Q6PCD4
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表达区间
S23-E703
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氨基酸序列
SSTTKCTVSHEVADCSHLKLTQVPDDLPTNITVLNLTHNQLRRLPAANFTRYSQLTSLDVGFNTISKLEPELCQKLPMLKVLNLQHNELSQLSDKTFAFCTNLTELHLMSNSIQKIKNNPFVKQKNLITLDLSHNGLSSTKLGTQVQLENLQELLLSNNKIQALKSEELDIFANSSLKKLELSSNQIKEFSPGCFHAIGRLFGLFLNNVQLGPSLTEKLCLELANTSIRNLSLSNSQLSTTSNTTFLGLKWTNLTMLDLSYNNLNVVGNDSFAWLPQLEYFFLEYNNIQHLFSHSLHGLFNVRYLNLKRSFTKQSISLASLPKIDDFSFQWLKCLEHLNMEDNDIPGIKSNMFTGLINLKYLSLSNSFTSLRTLTNETFVSLAHSPLHILNLTKNKISKIESDAFSWLGHLEVLDLGLNEIGQELTGQEWRGLENIFEIYLSYNKYLQLTRNSFALVPSLQRLMLRRVALKNVDSSPSPFQPLRNLTILDLSNNNIANINDDMLEGLEKLEILDLQHNNLARLWKHANPGGPIYFLKGLSHLHILNLESNGFDEIPVEVFKDLFELKIIDLGLNNLNTLPASVFNNQVSLKSLNLQKNLITSVEKKVFGPAFRNLTELDMRFNPFDCTCESIAWFVNWINETHTNIPELSSHYLCNTPPHYHGFPVRLFDTSSCKDSAPFE
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蛋白长度
Partial
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分子量
90-130 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TLR3, or Toll-like receptor 3, is a critical component of the innate immune system, primarily responsible for recognizing double-stranded RNA, which is often associated with viral infections. Research into TLR3 recombinant proteins has gained momentum due to their potential therapeutic applications in infectious diseases and cancer. Studies show that TLR3 activation can enhance the immune response by inducing the production of pro-inflammatory cytokines and type I interferons, making it a key player in antiviral defenses. Additionally, the ability of TLR3 to modulate immune responses presents opportunities for vaccine development and adjuvant design. Recombinant TLR3 proteins facilitate detailed studies of its functional roles in immune signaling pathways, and they serve as valuable tools for exploring the receptor's interactions with ligands and downstream signaling molecules. Understanding the structure-function relationship of TLR3 through recombinant technology can provide insights into its role in various pathologies, including chronic inflammatory conditions and tumor immunology. As researchers explore the therapeutic potentials of TLR3, recombinant proteins are critical for elucidating mechanisms of action, developing TLR3-based therapies, and ultimately improving health outcomes in viral diseases and beyond.
