Analytical Data
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基因名
IL-6R alpha
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简介
IL-6R alpha is a subunit alpha of IL-6 receptors, also shared by other interleukin receptors. IL-6R alpha acts as IL-6 agonist and involves in JAK/STAT, MAPK, and Akt signaling pathway[1][2]. IL-6R alpha, Mouse consists of 460 amino acids (M1-R460) with two fibronectin type-III-like domains contained in the N-terminal part (109-214 a.a, 215-313 a.a). Soluble IL-6R (sIL-6R) can be detected in the cerebrospinal fluid, conducts trans signaling by binding IL-6 and dimerized gp130, and exhibits a immune tissue expression property. IL-6R alpha Protein, Mouse (HEK293, His) is soluble form and produced in HEK293 cells with a C-Terminal His-tag.
- Application
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生物活性
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 3.014 ng/mL in the presence of 2 ng/mL recombinant mouse IL-6, corresponding to a specific activity is 3.32×105 units/mg. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 3.014 ng/mL in the presence of 2 ng/mL recombinant mouse IL-6, corresponding to a specific activity is 3.32×105 units/mg.
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别名
Interleukin-6 receptor subunit alpha; IL-6R-alpha; IL-6R 1; sIL6R; CD126
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种属
Mouse
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
P22272
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表达区间
L20-P364
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氨基酸序列
LVLGSCRALEVANGTVTSLPGATVTLICPGKEAAGNVTIHWVYSGSQNREWTTTGNTLVLRDVQLSDTGDYLCSLNDHLVGTVPLLVDVPPEEPKLSCFRKNPLVNAICEWRPSSTPSPTTKAVLFAKKINTTNGKSDFQVPCQYSQQLKSFSCQVEILEGDKVYHIVSLCVANSVGSKSSHNEAFHSLKMVQPDPPANLVVSAIPGRPRWLKVSWQHPETWDPSYYLLQFQLRYRPVWSKEFTVLLLPVAQYQCVIHDALRGVKHVVQVRGKEELDLGQWSEWSPEVTGTPWIAEPRTTPAGILWNPTQVSVEDSANHEDQYESSTEATSVLAPVQESSSMSLP
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蛋白长度
Extracellular Domain
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分子量
50-65 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Interleukin-6 receptor alpha (IL-6Rα) is a crucial component of the interleukin-6 (IL-6) signaling pathway, which plays a significant role in immune response, inflammation, and hematopoiesis. Dysregulation of IL-6 and its receptor is linked to various disease conditions, including autoimmune disorders, chronic inflammatory diseases, and certain types of cancer. The study of IL-6Rα, particularly the development and application of recombinant proteins, has garnered attention in both therapeutic and diagnostic contexts. Recombinant IL-6Rα proteins can be utilized to better understand the functional mechanisms of IL-6 signaling and to explore potential therapeutic interventions. For example, monoclonal antibodies targeting IL-6Rα, such as tocilizumab, have shown promise in treating diseases like rheumatoid arthritis and cytokine release syndrome. Moreover, the investigation of IL-6Rα's role in signaling cascades contributes to the understanding of its involvement in various pathological conditions, potentially leading to the identification of new therapeutic targets. Recent advancements in recombinant protein technology have enabled the generation of high-quality IL-6Rα proteins, facilitating detailed studies on its structure, function, and interactions with other signaling molecules. Thus, research on IL-6Rα recombinant proteins is pivotal for elucidating the complexities of IL-6-related pathways and developing innovative strategies to combat related diseases.
