Analytical Data
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Gene name
IL-4R alpha
- Application
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Biological Activity
Measured by its ability to inhibit IL-4-dependent proliferation of TF 1 human erythroleukemic cells. The ED50 for this effect is ≤18.24 ng/mL in the presence 0.2 ng/mL of Recombinant Human IL-4, corresponding to a specific activity is ≥5.482×104 units/mg. Measured by its ability to inhibit IL-4-dependent proliferation of TF 1 human erythroleukemic cells. The ED50 for this effect is 18.24 ng/mL in the presence 0.2 ng/mL of Recombinant Human IL‑4, corresponding to a specific activity is 5.482×104 units/mg.
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Alternative Names
Interleukin-4 receptor subunit alpha; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; CD124
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Species
Canine
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
XP_022275636.1
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Expression Region
G2-Q230
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AA Sequence
GRLCSGLTFPVSCLVLVWVASSGSVKVLHEPSCFSDYISTSVCQWKMDHPTNCSAELRLSYQLDFMGSENHTCVPENREDSVCVCSMPIDDAVEADVYQLDLWAGQQLLWSGSFQPSKHVKPRTPGNLTVHPNISHTWLLMWTNPYPTENHLHSELTYMVNVSNDNDPEDFKVYNVTYMGPTLRLAASTLKSGASYSARVRAWAQTYNSTWSDWSPSTTWLNYYEPWEQ
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Protein Length
Partial
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Molecular Weight
40-60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Interleukin-4 receptor alpha (IL-4Rα) is a crucial component of the immune response, mediating effects of the cytokines IL-4 and IL-13, which are key players in allergy and asthma development. Overactivation of the IL-4Rα pathway can lead to exaggerated allergic reactions, making it a potential therapeutic target for allergic diseases and asthma. Recombinant IL-4Rα protein studies are aimed at understanding the receptor's structure, function, and signaling mechanisms, which can provide insights into how IL-4 and IL-13 influence immune responses. By developing and characterizing IL-4Rα recombinant proteins, researchers aim to elucidate its role in T-helper cell differentiation and regulation of immune responses. Furthermore, these proteins can serve as tools for drug discovery and the development of monoclonal antibodies that inhibit IL-4Rα signaling, offering new strategies for treating allergic conditions. Overall, investigating IL-4Rα through recombinant technology holds promise for advancing our understanding of immune regulation and identifying novel therapeutic approaches for immune-mediated diseases.
