Analytical Data
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基因名
Jagged-1/JAG1
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简介
Jagged-1/JAG1 protein is a ligand of multiple Notch receptors, mediates Notch signaling, affects cell fate decisions during hematopoiesis and enhances angiogenesis. It functions throughout mammalian cardiovascular development, regulates myoblast differentiation, and participates in the complex Notch signaling network through interactions with NOTCH1, NOTCH2, and NOTCH3. Jagged-1/JAG1 Protein, Rat (HEK293, His) is the recombinant rat-derived Jagged-1/JAG1 protein, expressed by HEK293.
- Application
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生物活性
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1.264 μg/mL, corresponding to a specific activity is 791.1392 units/mg. Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1.264 μg/mL, corresponding to a specific activity is 791.1392 units/mg.
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别名
AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1
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种属
Rat
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 90% as determined by reducing SDS-PAGE.
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蛋白编号
Q63722
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表达区间
S32-D1067, with substitutions GGARN56-60AE and DR63-64TLVRPY
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蛋白长度
Partial
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分子量
125-130 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Jagged-1 (JAG1) is a critical ligand in the Notch signaling pathway, which plays a vital role in various biological processes, including cell differentiation, proliferation, and apoptosis. Dysregulation of the Notch pathway, particularly involving JAG1, is implicated in numerous diseases, including cancer and developmental disorders. JAG1 interacts with Notch receptors on adjacent cells, triggering signaling cascades that influence cellular fate. Research into recombinant JAG1 protein focuses on understanding its structure-function relationship and its role in mediating intercellular communications. Recombinant JAG1 can be produced using various expression systems, enabling detailed studies on its interaction with Notch receptors and downstream signaling effects. Investigating the biochemical properties of JAG1, including its binding affinity and signaling efficacy, may provide insights into therapeutic targets for conditions associated with Notch signaling dysregulation. Additionally, recombinant JAG1 has potential applications in regenerative medicine and tissue engineering, as it could enhance cell survival and differentiation in therapeutic settings. The production and characterization of JAG1, therefore, represent a significant area of interest for advancing our understanding of Notch signaling and its implications in health and disease.
