Analytical Data
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Gene name
SPANX-N3
- Application
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Alternative Names
Nuclear-associated protein SPAN-Xn3; SPANX family member N3; SPANX-N3; SPANXN3; Sperm protein associated with the nucleus on the X chromosome N3; SPXN3_HUMAN
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5MJ09
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Expression Region
1-141 aa
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AA Sequence
MEQPTSSTNG EKTKSPCESN NKKNDEMQEV PNRVLAPEQS LKKTKTSEYP IIFVYYLRKG KKINSNQLEN EQSQENSINP IQKEEDEGVD LSEGSSNEDE DLGPCEGPSK EDKDLDSSEG SSQEDEDLGL SEGSSQDSGE D
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Molecular Weight
15.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SPANX-N3 is a member of the SPANX family of proteins, which are expressed primarily in human testes and are classified as cancer/testis antigens (CTAs). These proteins are of significant interest due to their restricted expression patterns, being predominantly found in germ cells and certain malignancies, making them potential targets for immunotherapy. Research into SPANX-N3 has gained momentum in recent years as it is linked with various tumors, including melanoma and breast cancer. Because CTAs like SPANX-N3 are typically immunogenic and can evoke an immune response, scientists are exploring their utility in developing targeted cancer vaccines. Understanding the structure, function, and regulation of SPANX-N3 can provide insights into its role in tumorigenesis and immune evasion, and also help in designing novel therapeutic strategies that harness the immune system to target cancer cells expressing these antigens. Additionally, the study of SPANX-N3 contributes to a broader understanding of testis-specific proteins and their potential implications in fertility and reproductive health. Overall, SPANX-N3 presents a promising avenue for research in both cancer biology and immunology, as scientists aim to leverage these insights for innovative cancer treatment modalities.











