Analytical Data
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Gene name
iolG
- Application
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Alternative Names
iolG；idh；Inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P26935
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Expression Region
1-344aa
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AA Sequence
MSLRIGVIGTGAIGKEHINRITNKLSGAEIVAVTDVNQEAAQKVVEQYQLNATVYPNDDSLLADENVDAVLVTSWGPAHESSVLKAIKAQKYVFCEKPLATTAEGCMRIVEEEIKVGKRLVQVGFMRRYDSGYVQLKEALDNHVIGEPLMIHCAHRNPTVGDNYTTDMAVVDTLVHEIDVLHWLVNDDYESVQVIYPKKSKNALPHLKDPQIVVIETKGGIVINAEIYVNCKYGYDIQCEIVGEDGIIKLPEPSSISLRKEGRFSTDILMDWQRRFVAAYDVEIQDFIDSIQKKGEVSGPTAWDGYIAAVTTDACVKAQESGQKEKVELKEKPEFYQSFTTVQN
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Molecular Weight
54.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of iolG recombinant protein is rooted in the broader context of understanding the metabolic pathways involved in the utilization of inositol, a six-carbon sugar alcohol with significant roles in cellular signaling and metabolism. iolG encodes an enzyme involved in the inositol catabolic pathway, specifically facilitating the conversion of myo-inositol to D-xylulose-5-phosphate. This pathway is crucial for various organisms, including bacteria and fungi, as it aids in energy production and carbon metabolism. Given the rising interest in microbial bioengineering and synthetic biology, understanding the iolG gene and its protein product has garnered attention, particularly for applications in biotechnology and bioconversion processes. Research on iolG recombinant protein not only enhances our knowledge of inositol metabolism but also provides potential avenues for developing biofuels and bioplastics, contributing to environmental sustainability. Moreover, the study of iolG can lead to insights into microbial growth and survival mechanisms in nutrient-limited environments, thereby advancing our understanding of microbial ecology and evolution. Overall, the exploration of iolG recombinant protein stands at the intersection of fundamental biochemistry and practical applications, making it a valuable focus for researchers aiming to harness microbial capabilities for innovative solutions in various fields.
