Analytical Data
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Gene name
PYC1
- Application
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Alternative Names
PYC1；ASC2；ASCI；POP1；Pyrin domain-containing Protein 1
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P11154
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Expression Region
18-470aa
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AA Sequence
EKNKILVANRGEIPIRIFRTAHELSMQTVAIYSHEDRLSTHKQKADEAYVIGEVGQYTPVGAYLAIDEIISIAQKHQVDFIHPGYGFLSENSEFADKVVKAGITWIGPPAEVIDSVGDKVSARNLAAKANVPTVPGTPGPIETVEEALDFVNEYGYPVIIKAAFGGGGRGMRVVREGDDVADAFQRATSEARTAFGNGTCFVERFLDKPKHIEVQLLADNHGNVVHLFERDCSVQRRHQKVVEVAPAKTLPREVRDAILTDAVKLAKECGYRNAGTAEFLVDNQNRHYFIEINPRIQVEHTITEEITGIDIVAAQIQIAAGASLPQLGLFQDKITTRGFAIQCRITTEDPAKNFQPDTGRIEVYRSAGGNGVRLDGGNAYAGTIISPHYDSMLVKCSCSGSTYEIVRRKMIRALIEFRIRGVKTNIPFLLTLLTNPVFIEGTYWTTFIDDTPQ
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Molecular Weight
52.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of the PYC1 recombinant protein is rooted in the growing interest in protein engineering and its applications in biotechnology and medicine. PYC1, or pyruvate carboxylase 1, is an enzyme that plays a crucial role in gluconeogenesis and amino acid biosynthesis, particularly in the metabolism of various organisms. Its significance lies in its participation in the conversion of pyruvate to oxaloacetate, a key step in metabolic pathways that support energy production and carbon metabolism. Researchers have identified PYC1 as a potential target for therapeutic interventions in metabolic disorders and cancer, given its central role in regulating metabolic flux. The recombinant form of PYC1 allows scientists to study its structural and functional properties in detail, facilitating the exploration of enzyme kinetics, stability, and interaction with various substrates and inhibitors. Furthermore, advancements in recombinant DNA technology have made it possible to produce large quantities of PYC1, paving the way for its use in functional assays and potential industrial applications, such as biofuel production and biosynthesis of valuable biochemicals. Overall, the investigation of PYC1 recombinant protein not only enhances our understanding of its biochemical functions but also contributes to the development of innovative approaches in metabolic engineering and therapeutic design.
