Analytical Data
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Gene name
PDGF-AA
- Application
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Biological Activity
The ED50 is <50 ng/mL as measured by 3T3 cells. Measured in a cell proliferation assay using Balb/c 3T3 cells . The ED50 for this effect is 4.207 ng/mL, corresponding to a specific activity is 2.377×105units/mg.
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Alternative Names
rMuPDGF-AA; PDGF-1; Pdgfa
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Species
Mouse
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P20033
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Expression Region
S87-T211
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Protein Length
Full Length of Mature Protein
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Molecular Weight
17-28.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Platelet-derived growth factor AA (PDGF-AA) is a significant protein involved in various biological processes, particularly in cell growth, development, and tissue repair. As a member of the PDGF family, PDGF-AA is primarily secreted by platelets and plays a crucial role in the regulation of cell proliferation and differentiation, particularly in connective tissues. Its mechanisms of action are mediated through specific receptors, leading to various downstream signaling pathways that influence angiogenesis, wound healing, and fibrogenesis. Recent studies have highlighted PDGF-AA's implications in pathological conditions such as fibrosis, cancer, and vascular diseases, making it a target of interest for therapeutic interventions. Recombinant PDGF-AA protein has garnered attention in research settings for its potential applications in regenerative medicine, particularly in enhancing tissue repair and modulating fibrotic processes. The ability to produce PDGF-AA in a recombinant form allows for detailed studies of its biological functions and interactions within the extracellular matrix, paving the way for potential clinical applications. Ongoing research aims to elucidate its role in cellular mechanisms and explore its therapeutic efficacy in various disease models, emphasizing the importance of PDGF-AA in both fundamental biology and translational medicine.
