Analytical Data
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Gene name
PDGF-DD
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简介
PDGF-DD protein is an important growth factor that controls embryonic development, cell proliferation, migration, survival and chemotaxis, and has a potent mitogenic effect on mesenchymal cells. It plays a key role in wound healing and has carcinogenic potential, contributing to tumor formation. PDGF-DD Protein, Mouse (His-SUMO) is the recombinant mouse-derived PDGF-DD protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Application
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Biological Activity
Measured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells. The ED50 for this effect is 62.82 ng/mL, corresponding to a specific activity is 1.59×104 units/mg. Measured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells. The ED50 for this effect is 62.82 ng/mL, corresponding to a specific activity is 1.59×104 units/mg.
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Alternative Names
Pdgfd; Scdgfb; Platelet-derived growth factor D; PDGF-D; Spinal cord-derived growth factor B; SCDGF-B
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Species
Mouse
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Source
E. coli
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Tag
N-SUMO;N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q925I7-1
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Expression Region
T24-R370
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AA Sequence
TPQRASIKALRNANLRRDESNHLTDLYQREENIQVTSNGHVQSPRFPNSYPRNLLLTWWLRSQEKTRIQLSFDHQFGLEEAENDICRYDFVEVEEVSESSTVVRGRWCGHKEIPPRITSRTNQIKITFKSDDYFVAKPGFKIYYSFVEDFQPEAASETNWESVTSSFSGVSYHSPSITDPTLTADALDKTVAEFDTVEDLLKHFNPVSWQDDLENLYLDTPHYRGRSYHDRKSKVDLDRLNDDVKRYSCTPRNHSVNLREELKLTNAVFFPRCLLVQRCGGNCGCGTVNWKSCTCSSGKTVKKYHEVLKFEPGHFKRRGKAKNMALVDIQLDHHERCDCICSSRPPR
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Protein Length
Full Length of Isoform-1 Mature Protein
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Molecular Weight
66 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Platelet-derived growth factor-DD (PDGF-DD) is a member of the PDGF family, which plays a crucial role in various biological processes, including cell growth, survival, and tissue repair. It primarily acts as a potent mitogen for mesenchymal and other cell types, influencing the migration and proliferation of these cells. Research has indicated that PDGF-DD is involved in pathological conditions such as fibrosis, cancer progression, and wound healing, making it a significant target for therapeutic intervention. The recombinant expression of PDGF-DD allows for in-depth studies of its molecular mechanisms and interactions with specific receptors, such as PDGF receptor-beta (PDGFR-β). The elucidation of PDGF-DD's role can provide insights into its contribution to various diseases and enable the development of targeted therapies. Moreover, the understanding of its signaling pathways could reveal potential biomarkers for diagnosing and monitoring diseases associated with PDGF-DD activity. Thus, the study of PDGF-DD recombinant protein is essential for advancing our knowledge of its biological functions and therapeutic implications in regenerative medicine and oncology.
