Analytical Data
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Gene name
PDGF-AA
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简介
PDGF-AA protein is a key growth factor that serves as an effective mitogen for mesenchymal cells, coordinates embryonic development, and affects cell proliferation, migration, survival, and chemotaxis. It plays an important role in alveolar septal formation, gastrointestinal tract development, interstitial cell maturation, spermatogenesis, oligodendrocyte development, and myelination of the spinal cord and cerebellum. PDGF-AA Protein, Rat (P.pastoris, His) is the recombinant rat-derived PDGF-AA protein, expressed by P. pastoris , with N-His labeled tag.
- Application
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Alternative Names
Platelet-derived growth factor subunit A; PDGF-1; PDGF-A; Rpa1
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Species
Rat
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Source
P. pastoris
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Tag
N-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P28576-1
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Expression Region
R86-K204
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Protein Length
Full Length of Isoform 1 Mature Protein
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Molecular Weight
17-22 kDa & 25-45 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Platelet-derived growth factor AA (PDGF-AA) is a significant protein involved in various biological processes, particularly in cell growth, development, and tissue repair. As a member of the PDGF family, PDGF-AA is primarily secreted by platelets and plays a crucial role in the regulation of cell proliferation and differentiation, particularly in connective tissues. Its mechanisms of action are mediated through specific receptors, leading to various downstream signaling pathways that influence angiogenesis, wound healing, and fibrogenesis. Recent studies have highlighted PDGF-AA's implications in pathological conditions such as fibrosis, cancer, and vascular diseases, making it a target of interest for therapeutic interventions. Recombinant PDGF-AA protein has garnered attention in research settings for its potential applications in regenerative medicine, particularly in enhancing tissue repair and modulating fibrotic processes. The ability to produce PDGF-AA in a recombinant form allows for detailed studies of its biological functions and interactions within the extracellular matrix, paving the way for potential clinical applications. Ongoing research aims to elucidate its role in cellular mechanisms and explore its therapeutic efficacy in various disease models, emphasizing the importance of PDGF-AA in both fundamental biology and translational medicine.
