Analytical Data
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基因名
Leptin
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简介
Leptin is a cytokine-like sugar secretory protein secreted by adipocytes that helps regulate energy balance by inhibiting food intake and increasing thermogenesis. Leptin can activate the JAK2-STAT3 and PI3K-AKT/mTOR pathways to regulate energy metabolism, prolong the QT interval via MAPK in the myocardium, and play a protective role in the gastric mucosa by inducing NO/CGRP release via the vagus nerve. Circulating CRP protein binds to leptin and blocks signal transduction, leading to obesity resistance. Leptin Protein, Human is a recombinant human Leptin protein expressed by E. coli without a tag.
- Application
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生物活性
1.The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2.0 ng/mL, corresponding to a specific activity of > 5.0 × 105 IU/mg. 2.Immobilized Mouse LEPR (C-10His) at 10 μg/mL (100 μL/well) can bind Leptin, Human. The ED50 is 17.15 ng/mL. 3.Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human Leptin R. The ED50 for this effect is ≤1.948 ng/mL, corresponding to a specific activity is ≥5.133×105 units/mg. Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human Leptin R. The ED50 for this effect is 1.948 ng/mL, corresponding to a specific activity is 5.133×105 units/mg.
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别名
rHuLeptin; Obesity protein; OB
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种属
Human
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
P41159
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表达区间
V22-C167
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蛋白长度
Full Length of Mature Protein
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分子量
13-16 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Leptin is a critical adipocyte-derived hormone that plays a pivotal role in regulating energy balance, appetite, and metabolism. Discovered in the mid-1990s, leptin is primarily involved in signaling the brain to inhibit hunger and promote energy expenditure, thereby contributing to body weight homeostasis. Dysregulation of leptin signaling can lead to obesity, metabolic syndrome, and related health issues. As a result, leptin has become a significant focus in biomedical research aimed at understanding these conditions. Recombinant leptin protein, produced through genetic engineering techniques, enables detailed studies into leptin's physiological effects and mechanisms of action. Such research has implications not only for basic science but also for developing therapeutic strategies for obesity and its complications. Understanding the structure-function relationship of leptin and its receptor interactions through recombinant forms may pave the way for novel treatment options, including leptin-based therapies. Moreover, these recombinant proteins can serve as valuable tools for studying leptin's role in various biological systems, validating its potential as a biomarker for obesity-related disorders.
