Analytical Data
-
Gene name
Fractalkine/CX3CL1
-
简介
Fractalkine/CX3CL1 protein is a versatile chemokine that acts as a ligand for CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. It regulates immune response, inflammation, adhesion, and chemotaxis. It activates integrins via CX3CR1-dependent and -independent pathways, binding to site 1 with CX3CR1 and site 2 without CX3CR1. Soluble Fractalkine/CX3CL1 attracts T-cells and monocytes, not neutrophils. Fractalkine/CX3CL1 Protein, Rat is the recombinant rat-derived Fractalkine/CX3CL1 protein, expressed by E. coli , with tag free.
- Application
-
Biological Activity
Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 5.736 ng/mL, corresponding to a specific activity is 1.74×10^5 U/mg. Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells.The ED50 this effect is 5.736 ng/mL,corresponding to a specific activity is 1.74×105 U/mg.
-
Alternative Names
Fractalkine; Cx3cl1; C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1; Acc1; Fkn; Scyd1
-
Species
Rat
-
Source
E. coli
-
Tag
Tag Free
-
Purity
Greater than 95% as determined by reducing SDS-PAGE.
-
Uniprot
O55145
-
Expression Region
L22-G100
-
Protein Length
Partial
-
Molecular Weight
11 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Fractalkine, also known as CX3CL1, is a unique chemokine that exists in both a soluble form and a membrane-bound form, allowing it to play a dual role in immune response and cell signaling. It primarily interacts with its receptor CX3CR1, which is expressed on various immune cells, including monocytes, T-cells, and microglia. The Fractalkine/CX3CL1 axis is crucial for regulating leukocyte adhesion and migration, particularly in inflammatory conditions. Research has demonstrated that this signaling pathway is involved in several pathophysiological processes, including neuroinflammation in neurological disorders, cardiovascular diseases, and cancer metastasis. Recombinant Fractalkine/CX3CL1 proteins are valuable tools in experimental studies aimed at understanding the molecular mechanisms underlying these diseases. By utilizing recombinant proteins, researchers can investigate how Fractalkine regulates immune cell behavior, the role of the CX3CR1 receptor in immune system modulation, and the therapeutic potential of targeting this pathway. The development of recombinant Fractalkine/CX3CL1 not only facilitates basic research but also contributes to the exploration of potential therapeutic strategies for diseases characterized by aberrant inflammation and immune responses. Consequently, studies involving recombinant Fractalkine are critical for elucidating its biological functions and implications in health and disease, paving the way for future clinical applications.
