Cat: PA1000-1879

Recombinant Human MAGED1 Protein,His

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Analytical Data

  • Gene name

    MAGED1

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    MAGED1;NRAGE;Melanoma-associated antigen D1

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9Y5V3

  • Expression Region

    504-760aa

  • AA Sequence

    MGSSHHHHHH SSGLVPRGSH MGSLRPSPNS RASQNPGAAQ PRDVALLQER ANKLVKYLML KDYTKVPIKR SEMLRDIIRE YTDVYPEIIE RACFVLEKKF GIQLKEIDKE EHLYILISTP ESLAGILGTT KDTPKLGLLL VILGVIFMNG NRASEAVLWE ALRKMGLRPG VRHPLLGDLR KLLTYEFVKQ KYLDYRRVPN SNPPEYEFLW GLRSYHETSK MKVLRFIAEV QKRDPRDWTA QFMEAADEAL DALDAAAAEA EARAEARTRM GIGDEAVSGP

  • Molecular Weight

    32 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

MAGED1 (Melanoma Associated Gene D1) is a member of the MAGED gene family, which has been implicated in various physiological and pathological processes, including tumorigenesis. Initially identified as a gene expressed in melanoma cells, MAGED1 has garnered interest due to its potential role in cancer biology, specifically its involvement in regulating cell proliferation and apoptosis. Recent studies suggest that MAGED1 may also contribute to the immune response and the modulation of tumor immune evasion. Given its expression in various tumors and its potential as a cancer biomarker, understanding the functional properties of MAGED1 through recombinant protein studies is crucial. This research focuses on characterizing the biochemical and biophysical properties of MAGED1 to elucidate its role in cancer and explore its potential as a therapeutic target. By producing recombinant MAGED1, researchers aim to investigate its interactions with other cellular proteins, assess its effects on cellular pathways, and evaluate its implications for diagnostic and therapeutic strategies in cancer treatment. Ultimately, this foundational work is vital for advancing our understanding of MAGED1's contributions to cancer and harnessing its potential in clinical applications.

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