Analytical Data
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Gene name
KLHL22
- Application
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Alternative Names
KLHL22Kelch-like protein 22
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q53GT1
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Expression Region
1-634aa
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AA Sequence
MAEEQEFTQLCKLPAQPSHPHCVNNTYRSAQHSQALLRGLLALRDSGILFDVVLVVEGRHIEAHRILLAASCDYFRGMFAGGLKEMEQEEVLIHGVSYNAMCQILHFIYTSELELSLSNVQETLVAACQLQIPEIIHFCCDFLMSWVDEENILDVYRLAELFDLSRLTEQLDTYILKNFVAFSRTDKYRQLPLEKVYSLLSSNRLEVSCETEVYEGALLYHYSLEQVQADQISLHEPPKLLETVRFPLMEAEVLQRLHDKLDPSPLRDTVASALMYHRNESLQPSLQSPQTELRSDFQCVVGFGGIHSTPSTVLSDQAKYLNPLLGEWKHFTASLAPRMSNQGIAVLNNFVYLIGGDNNVQGFRAESRCWRYDPRHNRWFQIQSLQQEHADLSVCVVGRYIYAVAGRDYHNDLNAVERYDPATNSWAYVAPLKREVYAHAGATLEGKMYITCGRRGEDYLKETHCYDPGSNTWHTLADGPVRRAWHGMATLLNKLYVIGGSNNDAGYRRDVHQVACYSCTSGQWSSVCPLPAGHGEPGIAVLDNRIYVLGGRSHNRGSRTGYVHIYDVEKDCWEEGPQLDNSISGLAACVLTLPRSLLLEPPRGTPDRSQADPDFASEVMSVSDWEEFDNSSED
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Molecular Weight
98.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KLHL22, a member of the Kelch-like protein family, has garnered attention in recent years due to its potential role in various biological processes, including cell differentiation, immune response, and cancer progression. This protein contains multiple Kelch repeats, which are involved in protein-protein interactions, suggesting that KLHL22 may function as a substrate adapter for E3 ubiquitin ligases, thereby regulating protein degradation and cellular signaling pathways. Research has indicated that KLHL22 is differentially expressed in various tissues and its expression levels may correlate with specific diseases, making it a promising candidate for understanding disease mechanisms. Studies have also highlighted its involvement in modulating the immune response, linking KLHL22 to autoimmune diseases and inflammatory processes. Furthermore, aberrations in KLHL22 expression or function have been implicated in tumorigenesis, identifying it as a potential target for therapeutic intervention. The generation of recombinant KLHL22 protein is crucial for elucidating its structure-function relationship, understanding its interaction with binding partners, and exploring its role in ubiquitination pathways. As such, ongoing research focusing on the characterization and functional analysis of KLHL22 is essential to unravel its biological significance and therapeutic potential in various diseases.
