Analytical Data
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Gene name
splD
- Application
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Alternative Names
splD；Serine protease SplD
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q2FXC5
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Expression Region
37-239aa
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AA Sequence
ENSVKLITNTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYKVTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPNGNKLQMYESTGKVLSVNGNIVTSDAVVQPGSSGSPILNSKREAIGVMYASDKPTGESTRSFAVYFSPEIKKFIADNLDK
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Molecular Weight
29.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of the splD recombinant protein stems from its critical role in bacterial pathogenesis, particularly in the context of certain phytopathogenic bacteria. SplD is part of a larger family of proteins involved in the secretion systems that facilitate the delivery of effector proteins into host cells, thereby modulating host responses and promoting disease. Understanding the structure and function of splD can provide insights into its mechanisms of action, which are crucial for the development of novel strategies for disease management and prevention. Additionally, efforts to produce and characterize splD as a recombinant protein are driven by the need for targeted research tools. By utilizing techniques such as protein purification and characterization, researchers aim to unravel the biochemical pathways influenced by splD and explore its potential as a target for antibacterial therapies. Given the increasing concern over antibiotic resistance, elucidating the role of splD could lead to innovative approaches in combating bacterial infections, particularly those caused by resistant strains. Thus, the exploration of splD not only contributes to fundamental microbiological knowledge but also has significant implications for agricultural sustainability and public health.
