Analytical Data
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基因名
HSD11b1
- Application
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别名
HSD11b1；HSD11；HSD11L；SDR26C1；11-beta-hydroxysteroid dehydrogenase 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P28845
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表达区间
1-292aa
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氨基酸序列
MAFMKKYLLPILGLFMAYYYYSANEEFRPEMLQGKKVIVTGASKGIGREM AYHLAKMGAHVVVTARSKETLQKVVSHCLELGAASAHYIAGTMEDMTFAE QFVAQAGKLMGGLDMLILNHITNTSLNLFHDDIHHVRKSMEVNFLSYVVL TVAALPMLKQSNGSIVVVSSLAGKVAYPMVAAYSASKFALDGFFSSIRKE YSVSRVNVSITLCVLGLIDTETAMKAVSGIVHMQAAPKEECALEIIKGGA LRQEEVYYDSSLWTTLLIRNPCRKILEFLYSTSYNMDRFINK
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分子量
58 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The enzyme 11β-hydroxysteroid dehydrogenase type 1 (HSD11B1) plays a pivotal role in the metabolism of glucocorticoids, converting inactive cortisone to its active form, cortisol. This enzymatic activity is crucial in various physiological processes, including the regulation of metabolism, immune response, and stress responses. Dysregulation of HSD11B1 has been associated with several metabolic disorders, such as obesity, type 2 diabetes, and hypertension. Consequently, understanding the functional properties of HSD11B1 is essential for exploring its potential as a therapeutic target. Researchers have developed recombinant forms of the HSD11B1 protein to investigate its structure, enzymatic activity, and interaction with various substrates and inhibitors. These studies provide valuable insights into the molecular mechanisms underlying glucocorticoid action and contribute to the development of novel interventions aimed at modulating HSD11B1 activity in metabolic diseases. By creating and characterizing this recombinant protein, scientists aim to pave the way for innovative treatments that could alleviate the health burden associated with glucocorticoid-related disorders.
