Analytical Data
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基因名
GPI7
- Application
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别名
DKFZp434A1810; DKFZp434C1712; DKFZp434M1131; FLJ20265; FLJ39925; GPI ethanolamine phosphate transferase 2; GPI7; GPI7 homolog; hGPI7; LAS21; MGC131903; Phosphatidylinositol glycan anchor biosynthesis class G
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q5H8A4
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表达区间
1-983aa
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氨基酸序列
MRLGSGTFATCCVAIEVLGIAVFLRGFFPAPVRSSARAEHGAEPPAPEPSAGASSNWTTLPPPLFSKVVIVLIDALRDDFVFGSKGVKFMPYTTYLVEKGASHSFVAEAKPPTVTMPRIKALMTGSLPGFVDVIRNLNSPALLEDSVIRQAKAAGKRIVFYGDETWVKLFPKHFVEYDGTTSFFVSDYTEVDNNVTRHLDKVLKRGDWDILILHYLGLDHIGHISGPNSPLIGQKLSEMDSVLMKIHTSLQSKERETPLPNLLVLCGDHGMSETGSHGASSTEEVNTPLILISSAFERKPGDIRHPKHVQQTDVAATLAIALGLPIPKDSVGSLLFPVVEGRPMREQLRFLHLNTVQLSKLLQENVPSYEKDPGFEQFKMSERLHGNWIRLYLEEKHSEVLFNLGSKVLRQYLDALKTLSLSLSAQVAQYDIYSMMVGTVVVLEVLTLLLLSVPQALRRKAELEVPLSSPGFSLLFYLVILVLSAVHVIVCTSAESSCYFCGLSWLAAGGVMVLASALLCVIVSVLTNVLVGGNTPRKNPMHPSSRWSELDLLILLGTAGHVLSLGASSFVEEEHQTWYFLVNTLCLALSQETYRNYFLGDDGEPPCGLCVEQGHDGATAAWQDGPGCDVLERDKGHGSPSTSEVLRGREKWMVLASPWLILACCRLLRSLNQTGVQWAHRPDLGHWLTSSDHKAELSVLAALSLLVVFVLVQRGCSPVSKAALALGLLGVYCYRAAIGSVRFPWRPDSKDISKGIIEARFVYVFVLGILFTGTKDLLKSQVIAADFKLKTVGLWEIYSGLVLLAALLFRPHNLPVLAFSLLIQTLMTKFIWKPLRHDAAEITVMHYWFGQAFFYFQGNSNNIATVDISAGFVGLDTYVEIPAVLLTAFGTYAGPVLWASHLVHFLSSETRSGSALSHACFCYALICSIPVFTYIVLVTSLRYHLFIWSVFSPKLLYEGMHLLITAAVCVFFTAMDQTRLTQS
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分子量
108 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GPI7, or Glycosylphosphatidylinositol (GPI) transamidase component 7, is an essential enzyme involved in the post-translational modification of proteins, specifically in the biosynthesis of GPI anchors. GPI anchors are critical for attaching proteins to the cell membrane, playing vital roles in various cellular processes such as signal transduction, adhesion, and immune response. The study of GPI7 recombinantly produced protein has gained attention due to its implications in cellular function and potential connections to various diseases, including cancer and infections by pathogens that exploit GPI-anchored proteins for their lifecycle. Utilizing recombinant DNA technology to produce GPI7 facilitates the exploration of its biochemical properties, interactions with other cellular components, and its role in the GPI biosynthetic pathway. Understanding GPI7's function could provide insights into fundamental biological processes and may lead to the development of therapeutic strategies targeting GPI-related disorders. Furthermore, the ability to produce GPI7 in a laboratory setting allows researchers to conduct detailed structural and functional analyses, paving the way for innovations in biotechnology and medicine. Potential applications of this research span from improving vaccine development to enhancing understanding of cell signaling pathways, highlighting the importance of GPI7 in cell biology and its broader implications in health and disease.
