Analytical Data
-
Gene name
nucB
- Application
-
Alternative Names
nucB；NEFA；Nucleobindin-2
-
Species
Mouse
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q02819
-
Expression Region
1-459aa
-
AA Sequence
MPTSVPRGAPFLLLPPLLMLSAVLAVPVDRAAPPQEDSQATETPDTGLYYHRYLQEVINVLETDGHFREKLQAANAEDIKSGKLSQELDFVSHNVRTKLDELKRQEVSRLRMLLKAKMDAKQEPNLQVDHMNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWKTVEMSPAYTEEELKRFEEELAAREAELNARAQRLSQETEALGRSQDRLEAQKRELQQAVLQMEQRKQQLQEQSAPPSKPDGQLQFRADTDDAPVPAPAGDQKDVPASEKKVPEQPPELPQLDSQHL
-
Molecular Weight
53.4 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The nucB gene, encoding a secreted nuclease, has garnered significant attention in the field of molecular biology due to its role in bacterial adaptations and interactions with host organisms. Found in various strains of *Bacillus*, especially *Bacillus subtilis*, nucB is implicated in the degradation of nucleic acids, which can aid in microbial competition by modulating the availability of genetic material in the environment. Research on recombinantly expressed NucB protein aims to elucidate its enzymatic mechanisms, substrate specificity, and potential applications in biotechnology and medicine. Additionally, understanding the structural and functional dynamics of NucB can provide insights into its evolutionary advantages and its role in pathogenicity for certain bacteria. Recombinant NucB holds promise in developing novel therapeutic strategies, including antimicrobial treatments and biocontrol agents in agriculture, to combat bacterial infections and enhance plant health. The study of NucB is thus not only significant for basic microbiological research but also has far-reaching implications for applied sciences and public health.
