Analytical Data
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Gene name
ENA-78/CXCL5
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简介
CXCL5, also known as neutrophil activating peptide 78 (ENA‐78), is a CXC chemokine containing ELR motif. CXCL5 promotes angiogenesis through interaction with its specific receptor CXCR2. CXCL5 is expressed by many immune cells, such as macrophages, eosinophils, and non-immune cells including mesothelial cells, and fibroblasts.CXCL5/CXCR2 axis not only contributes to the recruitment of neutrophils but also regulates the function of neutrophils in melanoma[1][2]. ENA-78/CXCL5 Protein, Human is produced in E.coil, and consists of 70 amino acids (R45-N114).
- Application
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Biological Activity
1.The ED50 is <50 ng/mL as measured by CHO-K1/Gα15/hCXCR2 cells (human Gα15 and human CXCR2 stably expressed in CHO-K1 cells). 2.Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 6.072 ng/mL, corresponding to a specific activity is 1.647×105 U/mg. Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 6.072 ng/mL, corresponding to a specific activity is 1.647×105 U/mg.
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Alternative Names
rHuENA-78/CXCL5; ENA78; SCYB5
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P42830
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Expression Region
R45-N114
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Protein Length
Partial
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Molecular Weight
8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ENA-78, also known as CXCL5, is a member of the CXC chemokine family and plays a pivotal role in the immune response, particularly in neutrophil recruitment and activation. It is primarily produced by various cell types, including epithelial cells, and is implicated in several inflammatory processes and diseases, such as chronic obstructive pulmonary disease (COPD), rheumatoid arthritis, and certain cancers. The understanding of ENA-78/CXCL5's role in these conditions underscores its potential as a biomarker and therapeutic target. Recent advancements in recombinant protein technology have enabled the production of ENA-78/CXCL5 in sufficient quantities for detailed biochemical and functional studies. Researchers are investigating the protein's structure-function relationship, signaling pathways, and interactions with its receptor (CXCR2), which are crucial for deciphering its role in health and disease. In addition, studies are exploring how manipulating ENA-78/CXCL5 levels can influence immune responses and inflammation, providing insights for developing new treatments. As a result, generating a recombinant form of ENA-78/CXCL5 is essential for both basic research and potential therapeutic applications, contributing to our understanding of its biological significance and therapeutic utilities in various pathological contexts.
